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RNA Capture-SELEX on Streptavidin Magnetic Beads

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Nucleic Acid Aptamers

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2570))

Abstract

SELEX has enabled the selection of aptamers, nucleic acids that can bind a defined ligand, in some cases with exceptionally high affinity and specificity. The SELEX protocol has been adapted many times to fit a variety of needs. This protocol describes such an adaptation, namely, RNA-Capture SELEX that we have used to successfully develop small molecule-binding RNA aptamers. Our proposed method specifically selects not only for excellent binding but also for conformational switching. In consequence, we found this SELEX method to be particularly suitable for identifying aptamers for further application in synthetic riboswitch engineering.

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Author information

Authors and Affiliations

  1. Department of Biology, Technical University of Darmstadt, Darmstadt, Germany

    Leon Kraus & Beatrix Suess

  2. Center for Synthetic Biology, Technical University of Darmstadt, Darmstadt, Germany

    Beatrix Suess

Authors
  1. Leon Kraus
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  2. Beatrix Suess
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Corresponding author

Correspondence to Beatrix Suess .

Editor information

Editors and Affiliations

  1. Life and Medical Sciences Institute (LIMES), University of Bonn, Bonn, Germany

    Günter Mayer

  2. Branch Bioanalytics and Bioprocesses, Fraunhofer Institute for Cell Therapy and Immunology, Potsdam, Germany

    Marcus M. Menger

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Kraus, L., Suess, B. (2023). RNA Capture-SELEX on Streptavidin Magnetic Beads. In: Mayer, G., Menger, M.M. (eds) Nucleic Acid Aptamers. Methods in Molecular Biology, vol 2570. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2695-5_5

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  • DOI: https://doi.org/10.1007/978-1-0716-2695-5_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2694-8

  • Online ISBN: 978-1-0716-2695-5

  • eBook Packages: Springer Protocols

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