Abstract
The transcription factor Foxp3/FOXP3 orchestrates regulatory T (Treg) cell development and function by interacting with numerous target genes and partner proteins. Functional analysis of naturally occurring or engineered Foxp3/FOXP3 mutations has provided important insights into how the complex Foxp3/FOXP3-centered molecular network operates. Here, we describe detailed protocols for retroviral transduction of murine primary conventional CD4+ T cells to determine the impacts of Foxp3 mutations on the Treg-cell-like phenotype and function conferred by Foxp3.
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Acknowledgments
This work was supported by MEXT KAKENHI (17H06626 to R.M., 18H04025 to S.H., 19H04801 to S.H., 19Â K16601 to R.M., 19Â K16684 to A.N.) and by AMED PRIME (JP22gm6210018 to S.H.).
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Nakajima, A., Murakami, R., Hori, S. (2023). Functional Analysis of Foxp3 and Its Mutants by Retroviral Transduction of Murine Primary CD4+ T Cells. In: Ono, M. (eds) Regulatory T-Cells. Methods in Molecular Biology, vol 2559. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2647-4_7
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DOI: https://doi.org/10.1007/978-1-0716-2647-4_7
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-2647-4
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