Analysis of Gut Microbiome Using Fecal Samples

Abstract

With the gut microbiome being implicated in many diseases and even offering the potential for future therapies, its study has been gaining interest over the years. However, to gain more in-depth insight and understanding of the impact of the microbiome on our health, it is critical to have accurate microbial data that is reproducible and comparable across studies. Variation in experimental techniques, particularly DNA extraction and sequencing methods, may result in variability and the inability to compare results. In this chapter, we describe in detail our microbiome analysis methodology focusing on stool samples.

Appendix A

• Subjects should peel the corner of the plastic sleeve to open and lift the cap off the Collection and Transport tube.

• The dual applicator tips should only touch the fecal material to avoid contamination.

• Fecal sample is collected by applying the dual swab against used toilet paper until the applicator tips are covered with fecal material. Then, without separating the two swabs, subjects should insert them inside the Collection and Transport tube until it is securely in place.

• A sample identification sticker should be completed and affixed to the tube.

• The tube should be placed into the plastic bag and sealed.

• Store at refrigerator temperature until delivery to the study site.