Abstract
High-throughput phenotyping platforms for growth chamber and greenhouse-grown plants enable nondestructive, automated measurements of plant traits including shape, aboveground architecture, length, and biomass over time. However, to establish these platforms, many of these methods require expensive equipment or phenotyping expertise. Here we present a relatively inexpensive and simple phenotyping method for imaging hundreds of small- to medium-sized growth chamber or greenhouse-grown plants with a digital camera. Using this method, we image hundreds of tomato plants in 1 day.
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References
Furbank RT, Tester M (2011) Phenomics – technologies to relieve the phenotyping bottleneck. Trends Plant Sci 16:635–644
Tardieu F, Cabrera-Bosquet L, Pridmore T et al (2017) Plant phenomics, from sensors to knowledge. Curr Biol 27:R770–R783
Araus JL, Kefauver SC (2018) Breeding to adapt agriculture to climate change: affordable phenotyping solutions. Curr Opin Plant Biol 45:237–247
Fahlgren N, Gehan MA, Baxter I (2015) Lights, camera, action: high-throughput plant phenotyping is ready for a close-up. Curr Opin Plant Biol 24:93–99
Simko I, Jimenez-Berni JA, Sirault XRR (2017) Phenomic approaches and tools for phytopathologists. Phytopathology 107:6–17
Perez-Sanz F, Navarro PJ, Egea-Cortines M (2017) Plant phenomics: an overview of image acquisition technologies and image data analysis algorithms. GigaScience 6:1–18
Atkinson JA, Pound MP, Bennett MJ et al (2019) Uncovering the hidden half of plants using new advances in root phenotyping. Curr Opin Biotechnol 55:1–8
Paez-Garcia A, Motes C, Scheible WR et al (2015) Root traits and phenotyping strategies for plant improvement. Plan Theory 4:334–355
Gehan MA, Fahlgren N, Abbasi A et al (2017) PlantCV v2: image analysis software for high-throughput plant phenotyping. PeerJ 5:e4088
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Caldwell, D., Iyer-Pascuzzi, A.S. (2022). A Straightforward High-Throughput Aboveground Phenotyping Platform for Small- to Medium-Sized Plants. In: Lorence, A., Medina Jimenez, K. (eds) High-Throughput Plant Phenotyping. Methods in Molecular Biology, vol 2539. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2537-8_5
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DOI: https://doi.org/10.1007/978-1-0716-2537-8_5
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2536-1
Online ISBN: 978-1-0716-2537-8
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