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Microfabricated Liquid Junction Capillary Electrophoresis–Mass Spectrometry Interface

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Capillary Electrophoresis-Mass Spectrometry

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2531))

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Abstract

Coupling of capillary electrophoresis (CE) with mass spectrometry (MS) represents a powerful combination for performing rapid, efficient, and sensitive analysis of a variety of compounds. Here we describe a construction, operation, and application of a microfabricated liquid junction CE-MS interface. The interface is designed as a microfabricated unit with an integrated liquid junction and electrospray tip made from polyimide, which is positioned in a plastic connection block securing the separation CE capillary and attachable to the CE instrument. The application was demonstrated by CE-MS analysis of dextran oligomers labeled by (2-aminoethyl)trimethylammonium (AETMA) salt.

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Acknowledgments

This work was supported by the Grant Agency of the Czech Republic (22-00236S), the Czech Academy of Sciences (MTA-19-06), and the Institute of Analytical Chemistry of the Czech Academy of Sciences (RVO: 68081715).

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Authors and Affiliations

  1. Institute of Analytical Chemistry of the Czech Academy of Sciences, Brno, Czech Republic

    Jana Krenkova & Frantisek Foret

Authors
  1. Jana Krenkova
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  2. Frantisek Foret
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Corresponding author

Correspondence to Jana Krenkova .

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Editors and Affiliations

  1. Aalen University, Aalen, Baden-Württemberg, Germany

    Christian Neusüß

  2. Aalen University, Aalen, Baden-Württemberg, Germany

    Kevin Jooß

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Krenkova, J., Foret, F. (2022). Microfabricated Liquid Junction Capillary Electrophoresis–Mass Spectrometry Interface. In: Neusüß, C., Jooß, K. (eds) Capillary Electrophoresis-Mass Spectrometry . Methods in Molecular Biology, vol 2531. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2493-7_4

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  • DOI: https://doi.org/10.1007/978-1-0716-2493-7_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2492-0

  • Online ISBN: 978-1-0716-2493-7

  • eBook Packages: Springer Protocols

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