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Localizing Total mRNA in Plant Cells

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The Nuclear Pore Complex

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2502))

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Abstract

Visualizing the location of the total cellular mRNA pool can be important to understand how different genes affect cellular physiology. Over the past decade researchers investigating RNA processing, nuclear transport and the function of the nuclear pore complex have used in situ hybridization protocol to visualize and quantify the accumulation of the total mRNA pool within the plant cell nucleus.

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References

  1. Javelle M, Marco CF, Timmermans M (2011) In situ hybridization for the precise localization of transcripts in plants. J Vis Exp 57:e3328. https://doi.org/10.3791/3328

    Article  CAS  Google Scholar 

  2. Hejátko J, Blilou I, Brewer PB, Friml J, Scheres B, Benková E (2006) In situ hybridization technique for mRNA detection in whole mount Arabidopsis samples. Nat Protoc 1:1939–1946

    Article  Google Scholar 

  3. Duncan S, Olsson TSG, Hartley M, Dean C, Rosa S (2016) A method for detecting single mRNA molecules in Arabidopsis thaliana. Plant Methods 12:13. https://doi.org/10.1186/s13007-016-0114-x

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Gong Z, Dong CH, Lee H, Zhu J, Xiong L, Gong D, Stevenson B, Zhu JK (2005) A DEAD box RNA helicase is essential for mRNA export and important for development and stress responses in Arabidopsis. Plant Cell 17:256–267

    Article  CAS  Google Scholar 

  5. Parry G, Ward S, Cernac A, Dharmasiri S, Estelle M (2006) The Arabidopsis SUPPRESSOR OF AUXIN RESISTANCE proteins are nucleoporins with an important role in hormone signaling and development. Plant Cell 18:1590–1603

    Article  CAS  Google Scholar 

  6. Lu Q, Tang X, Tian G, Wang F, Liu K, Nguyen V, Kohalmi SE, Keller WA, Tsang EW, Harada JJ, Rothstein SJ, Cui Y (2010) Arabidopsis homolog of the yeast TREX-2 mRNA export complex: components and anchoring nucleoporin. Plant J 61:259–270. https://doi.org/10.1111/j.1365-313X.2009.04048.x

    Article  CAS  PubMed  Google Scholar 

  7. MacGregor DR, Gould P, Foreman J, Griffiths J, Bird S, Page R, Stewart K, Steel G, Young J, Paszkiewicz K, Millar AJ, Halliday KJ, Hall AJ, Penfield S (2013) HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENES1 is required for circadian periodicity through the promotion of nucleo-cytoplasmic mRNA export in Arabidopsis. Plant Cell 25:4391–4404. https://doi.org/10.1105/tpc.113.114959

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Parry G (2014) Components of the Arabidopsis nuclear pore complex play multiple diverse roles in control of plant growth. J Exp Bot 65:6057–6067. https://doi.org/10.1093/jxb/eru346

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Gallemí M, Galstyan A, Paulišić S, Then C, Ferrández-Ayela A, Lorenzo-Orts L, Roig-Villanova I, Wang X, Micol JL, Ponce MR, Devlin PF, Martínez-García JF (2016) DRACULA2 is a dynamic nucleoporin with a role in regulating the shade avoidance syndrome in Arabidopsis. Development 143:1623–1631. https://doi.org/10.1242/dev.130211

    Article  CAS  PubMed  Google Scholar 

  10. Sørensen BB, Ehrnsberger HF, Esposito S, Pfab A, Bruckmann A, Hauptmann J, Meister G, Merkl R, Schubert T, Längst G, Melzer M, Grasser M, Grasser KD (2017) The Arabidopsis THO/TREX component TEX1 functionally interacts with MOS11 and modulates mRNA export and alternative splicing events. Plant Mol Biol 93:283–298. https://doi.org/10.1007/s11103-016-0561-9

    Article  CAS  PubMed  Google Scholar 

  11. Ehrnsberger HF, Pfaff C, Hachani I, Flores-Tornero M, Sørensen BB, Längst G, Sprunck S, Grasser M, Grasser KD (2019) The UAP56-interacting export factors UIEF1 and UIEF2 function in mRNA export. Plant Physiol 179:1525–1536. https://doi.org/10.1104/pp.18.01476

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Engler JA, Van Montagu M, Engler G (1994) Hybridization in situ of whole-mount messenger RNA in plants. Plant Mol Biol Rep 12:321–331

    Article  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. GARNet, School of Biosciences, Cardiff University, Cardiff, UK

    Geraint Parry

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  1. Geraint Parry
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Editors and Affiliations

  1. Department of Biosciences, Durham University, Durham, UK

    Martin W. Goldberg

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Parry, G. (2022). Localizing Total mRNA in Plant Cells. In: Goldberg, M.W. (eds) The Nuclear Pore Complex. Methods in Molecular Biology, vol 2502. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2337-4_7

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  • DOI: https://doi.org/10.1007/978-1-0716-2337-4_7

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2336-7

  • Online ISBN: 978-1-0716-2337-4

  • eBook Packages: Springer Protocols

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