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Proteoform Identification

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Liangliang Sun

    1. Department of Chemistry, Michigan State University, East Lansing, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Xiaowen Liu

    1. Deming Department of Medicine, School of Medicine, Tulane University, New Orleans, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2500)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Proteoforms and Proteoform Families: Past, Present, and Future

    • Lloyd M. Smith
    Pages 1-4

  3. Membrane Ultrafiltration-Based Sample Preparation Method and Sheath-Flow CZE-MS/MS for Top-Down Proteomics

    • Zhichang Yang, Liangliang Sun
    Pages 5-14

  4. Size Exclusion Chromatography Strategies and MASH Explorer for Large Proteoform Characterization

    • Timothy N. Tiambeng, Zhijie Wu, Jake A. Melby, Ying Ge
    Pages 15-30

  5. RPLC-RPLC-MS/MS for Proteoform Identification

    • Kellye A. Cupp-Sutton, Zhe Wang, Dahang Yu, Si Wu
    Pages 31-42

  6. Monolithic Materials-Based RPLC-MS for Proteoform Separation and Identification

    • Yu Liang, Lihua Zhang, Yukui Zhang
    Pages 43-53

  7. Capillary Isoelectric Focusing: Mass Spectrometry Method for the Separation and Online Characterization of Monoclonal Antibody Charge Variants at Intact and Subunit Levels

    • Jun Dai, Qiangwei Xia, Chengjie Ji
    Pages 55-65

  8. Proteoform Analysis and Construction of Proteoform Families in Proteoform Suite

    • Leah V. Schaffer, Michael R. Shortreed, Lloyd M. Smith
    Pages 67-81

  9. Top-Down Mass Spectrometry Data Analysis Using TopPIC Suite

    • In Kwon Choi, Xiaowen Liu
    Pages 83-103

  10. Accurate Proteoform Identification and Quantitation Using pTop 2.0

    • Rui-Xiang Sun, Rui-Min Wang, Lan Luo, Chao Liu, Hao Chi, Wen-Feng Zeng et al.
    Pages 105-129

  11. Proteoform Identification and Quantification Using Intact Protein Database Search Engine ProteinGoggle

    • Suideng Qin, Zhixin Tian
    Pages 131-144

  12. Mass Deconvolution of Top-Down Mass Spectrometry Datasets by FLASHDeconv

    • Kyowon Jeong, Jihyung Kim, Oliver Kohlbacher
    Pages 145-157

  13. Deconvolving Native and Intact Protein Mass Spectra with UniDec

    • Marius M. Kostelic, Michael T. Marty
    Pages 159-180

  14. Discovery of Unknown Posttranslational Modifications by Top-Down Mass Spectrometry

    • Jesse W. Wilson, Mowei Zhou
    Pages 181-199

  15. Determining Copper and Zinc Content in Superoxide Dismutase Using Electron Capture Dissociation Under Native Spray Conditions

    • Rachel Franklin, Michael Hare, Joseph S. Beckman
    Pages 201-210

  16. Surface-Induced Dissociation for Protein Complex Characterization

    • Sophie R. Harvey, Gili Ben-Nissan, Michal Sharon, Vicki H. Wysocki
    Pages 211-237

  17. Back Matter

    Pages 239-240
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Keywords

About this book

This volume discusses the latest mass spectrometry (MS)-based technologies for proteoform identification, characterization, and quantification. Some of the topics covered in this book include sample preparation, proteoform separation, proteoform gas-phase fragmentation, and bioinformatics tools for MS data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Proteoform Identification: Methods and Protocols is a valuable resource for researchers in both academia and the biopharmaceutical industry who are interested in proteoform analysis using MS.  


Editors and Affiliations

  • Department of Chemistry, Michigan State University, East Lansing, USA

    Liangliang Sun

  • Deming Department of Medicine, School of Medicine, Tulane University, New Orleans, USA

    Xiaowen Liu

Bibliographic Information

  • Book Title: Proteoform Identification

  • Book Subtitle: Methods and Protocols

  • Editors: Liangliang Sun, Xiaowen Liu

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2325-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2324-4Published: 04 June 2022

  • Softcover ISBN: 978-1-0716-2327-5Published: 05 June 2023

  • eBook ISBN: 978-1-0716-2325-1Published: 03 June 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 240

  • Number of Illustrations: 8 b/w illustrations, 74 illustrations in colour

  • Topics: Mass Spectrometry

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