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iPSC-Derived Micro-Heart Muscle for Medium-Throughput Pharmacology and Pharmacogenomic Studies

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Cardiac Tissue Engineering

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2485))

Abstract

Micro-heart muscle arrays enable medium-throughput experiments to model the cardiac response to a variety of environmental and pharmaceutical effects. Here, we describe stem cell culture maintenance, methods for successful cardiac differentiation, and formation of micro-heart muscle arrays for electrophysiology and molecular biology assays.

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Author information

Authors and Affiliations

  1. Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, MO, USA

    Daniel W. Simmons & Nathaniel Huebsch

  2. NSF Science and Technology Center for Engineering Mechanobiology, Washington University in St. Louis, St. Louis, MO, USA

    Daniel W. Simmons & Nathaniel Huebsch

  3. Center for Cardiovascular Research, Center for Regenerative Medicine, Center for Investigation of Membrane Excitability Diseases, Washington University in St. Louis, St. Louis, MO, USA

    Nathaniel Huebsch

Authors
  1. Daniel W. Simmons
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  2. Nathaniel Huebsch
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Corresponding author

Correspondence to Nathaniel Huebsch .

Editor information

Editors and Affiliations

  1. School of Engineering, Brown University, Providence, RI, USA

    Kareen L.K. Coulombe

  2. Department of Biomedical Engineering, Tufts University, Medford, MA, USA

    Lauren D. Black III

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Simmons, D.W., Huebsch, N. (2022). iPSC-Derived Micro-Heart Muscle for Medium-Throughput Pharmacology and Pharmacogenomic Studies. In: Coulombe, K.L., Black III, L.D. (eds) Cardiac Tissue Engineering. Methods in Molecular Biology, vol 2485. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2261-2_8

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  • DOI: https://doi.org/10.1007/978-1-0716-2261-2_8

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2260-5

  • Online ISBN: 978-1-0716-2261-2

  • eBook Packages: Springer Protocols

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