Abstract
This chapter describes a method for creating tubular cardiac tissue in vitro. Thick cardiac tissue in a tubular configuration is prepared by stacking cell sheets stepwise on the inner wall of a segment of small intestine, which functions as a blood vessel bed. The capillaries of the small intestinal segment are fed by an artery and drained by a vein. Therefore, perfusion culture of the cardiac tissue is achieved by continuously infusing culture medium into the arterial vessel that supplies the segment of small intestine. The aim of this technique is to fabricate tubular cardiac tissue that can function as a pump by sequentially implanting and culturing cardiac cell sheets on the inner wall of a perfused segment of small intestine.
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Acknowledgments
This research was supported by the “Development of innovative manufacturing technology for three-dimensional tissues and organs based on cell sheet engineering” from the Japan Agency for Medical Research and Development (AMED, https://www.amed.go.jp/en/index.html; grant no. JP17he0702249) and JSPS KAKENHI grant no. 19H04453. The authors thank OXMEDCOMMS (www.oxmedcomms.com) for writing assistance. We also acknowledge the assistance of Prof. Tatsuya Shimizu, Prof. Jun Homma, Dr. Kazunori Sano, Mr. Shogo Shimizu, Prof. Katsuhisa Matsuura, and Prof. Eiji Kobayashi.
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Sekine, H., Okano, T. (2022). Tubular Cardiac Tissue Bioengineered from Multi-Layered Cell Sheets for Use in the Treatment of Heart Failure. In: Coulombe, K.L., Black III, L.D. (eds) Cardiac Tissue Engineering. Methods in Molecular Biology, vol 2485. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2261-2_15
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DOI: https://doi.org/10.1007/978-1-0716-2261-2_15
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