Abstract
Metabolite profiling aiming at quantifying the metabolome of flowers is emerging as a suitable tool to understand the metabolic complexity of these reproductive organs and the associations between primary and secondary metabolites which characterize them. This chapter provides a general method for the combined analyses of primary and secondary metabolites via gas chromatography–mass spectrometry (GC-MS) and high-performance liquid chromatography–mass spectrometry (LC-MS) of flower samples. We describe the preparatory steps, the procedure of metabolites’ extraction and finally provide examples of data representation. The method described here can be applied to the analysis of metabolomes of entire flowers, as well as specific flower organs.
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Acknowledgments
The authors gratefully acknowledge the financial support of the Max Planck Society and Utah State University startup funding to M.B.
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Borghi, M., Perez de Souza, L., Fernie, A.R. (2022). Measurement of Flower Metabolite Concentrations Using Gas Chromatography–Mass Spectrometry and High-Performance Liquid Chromatography–Mass Spectrometry. In: Lambing, C. (eds) Plant Gametogenesis. Methods in Molecular Biology, vol 2484. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2253-7_1
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DOI: https://doi.org/10.1007/978-1-0716-2253-7_1
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