Abstract
In addition to diet quality and quantity, the “timing” of food intake recently emerged as a third key parameter in nutritional and metabolic health. The link between nutrition timing and metabolic homeostasis is in part due to the regulation of daily feeding:fasting cycles and metabolic pathways by the circadian clock. Preclinical feeding regimen studies in rodents are invaluable to further define the modalities of this relationship and get a better understanding of its mechanistic underpinnings. Time-restricted feeding (TRF) and caloric restriction (CR) are examples of feeding regimen at the crossroads of metabolic and circadian regulation. Here we propose methods to implement TRF and CR highlighting the parameters that are relevant to the study of circadian and metabolic health. We also provide methods to determine their impact on the output of the circadian clock by analyzing diurnal expression profiles using 24 h time-series collection as well as their impact on metabolic homeostasis using a glucose tolerance test (GTT).
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Acknowledgments
I would like to acknowledge the numerous outstanding scientists from every stage of the scientific career path in the Panda and Chaix’s lab who have made it possible for me to run TRF experiments for almost 10 years. I also would like to thank Patrick-Simon Welz for careful and helpful reading and editing of this chapter. This chapter is dedicated to Drs. Paolo Sassone-Corsi and Michael E. Hughes for their influential role in my scientific training as a chronobiologist. AC is supported by grants from the National Institutes of Health (NIH) R01 AG065993 and from the American Heart Association (AHA) 18CDA34110292.
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Chaix, A. (2022). Time-Restricted Feeding and Caloric Restriction: Two Feeding Regimens at the Crossroad of Metabolic and Circadian Regulation. In: Solanas, G., Welz, P.S. (eds) Circadian Regulation. Methods in Molecular Biology, vol 2482. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2249-0_22
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DOI: https://doi.org/10.1007/978-1-0716-2249-0_22
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