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Notch Signaling Research

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Dongyu Jia

    1. Department of Biology, Georgia Southern University, Statesboro, USA

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2472)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xiii
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  2. Using the CRISPR/Cas9 System for Dissection of Functional Sites of the Notch Gene in Drosophila melanogaster

    • Oleg V. Andreyenkov, Elena I. Volkova, Natalya G. Andreyenkova, Sergey A. Demakov
    Pages 1-14

  3. Screening Mutants by Single Fly Genomic PCR

    • Usha Nagarajan, Marios Georgiou
    Pages 15-25

  4. Generation of Properly Folded Epidermal Growth Factor-Like (EGF) Repeats and Glycosyltransferases Enables In Vitro O-Glycosylation

    • Chenyu Ma, Yohei Tsukamoto, Hideyuki Takeuchi
    Pages 27-38

  5. Use of FLP/FRT System to Screen for Notch Signaling Regulators in the Drosophila Wing

    • Dongqing Mo, Jie Shen, Junzheng Zhang
    Pages 39-48

  6. High-Throughput Analysis to Identify Activators of Notch Signaling

    • Rachael Guenter, Jacob Eide, Herbert Chen, J. Bart Rose, Renata Jaskula-Sztul
    Pages 49-56

  7. Artificial Notch Signaling Activation Method Using Immobilized Ligand Beads

    • Takamasa Mizoguchi, Hikaru Handa, Shuhei Omaru, Motoyuki Itoh
    Pages 57-66

  8. Mammalian NOTCH Receptor Activation and Signaling Protocols

    • María-Luisa Nueda, Victoriano Baladrón
    Pages 67-82

  9. Somatic Clonal Analyses Using FLP/FRT and MARCM System to Understand Notch Signaling Mechanism and Its Regulation

    • Vartika Sharma, Nalani Sachan, Mousumi Mutsuddi, Ashim Mukherjee
    Pages 83-94

  10. Analyzing the Interaction of RBPJ with Mitotic Chromatin and Its Impact on Transcription Reactivation upon Mitotic Exit

    • Kostiantyn Dreval, Robert J. Lake, Hua-Ying Fan
    Pages 95-108

  11. Assessing the Roles of Potential Notch Signaling Components in Instructive and Permissive Pathways with Two Drosophila Pericardial Reporters

    • Manoj Panta, Andrew J. Kump, Kristopher R. Schwab, Shaad M. Ahmad
    Pages 109-130

  12. Image-Based Single-Molecule Analysis of Notch-Dependent Transcription in Its Natural Context

    • ChangHwan Lee, Tina Lynch, Sarah L. Crittenden, Judith Kimble
    Pages 131-149

  13. An Automatic Stage Identification MATLAB Tool to Reveal Notch Expression Pattern in Drosophila Egg Chambers

    • Lily Paculis, Qiuping Xu, Qian Xie, Dongyu Jia
    Pages 151-157

  14. Employing the CRISPR Technology for Studying Notch Signaling in the Male Gonad of Drosophila melanogaster

    • Cordula Schulz
    Pages 159-172

  15. Immunolocalization of Notch Signaling in Mouse Preimplantation Embryos

    • Elisabete Silva, Patrícia Diniz, Alexandre Trindade, Mariana Batista, Ana Torres, António Duarte et al.
    Pages 173-185

  16. Studying the NOTCH Signaling Pathway Activation in Kidney Biopsies

    • Laura Márquez-Expósito, Carolina Lavoz, Elena Cantero-Navarro, Raúl R. Rodrigues-Diez, Sergio Mezzano, Marta Ruiz-Ortega
    Pages 187-196

  17. Exosomes as Carriers for Notch Molecules

    • Guya Diletta Marconi, Francesca Diomede, Oriana Trubiani, Cristina Porcheri, Thimios A. Mitsiadis
    Pages 197-208

  18. In Vivo and Ex Vivo Experimental Approach for Studying Functional Role of Notch in Pulmonary Vascular Disease

    • Pritesh P. Jain, Susumu Hosokawa, Aleksandra Babicheva, Tengteng Zhao, Jiyuan Chen, Patricia A. Thistlethwaite et al.
    Pages 209-220

  19. Metastasis Model to Test the Role of Notch Signaling in Prostate Cancer

    • Shiqin Liu, En-chi Hsu, Michelle Shen, Merve Aslan, Tanya Stoyanova
    Pages 221-233

  20. Functional Studies of Genetic Variants Associated with Human Diseases in Notch Signaling-Related Genes Using Drosophila

    • Sheng-An Yang, Jose L. Salazar, David Li-Kroeger, Shinya Yamamoto
    Pages 235-276
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Keywords

About this book

This detailed volume explores the large research area of Notch signaling studies in a variety of model organisms. The collected research methods and protocols include the dissection of Notch functional sites, Notch regulators, Notch activity reporters and analysis, roles of Notch in development and diseases, wet and dry lab tools and studies, and bioinformatics analysis. This book particularly emphasizes on the exploration of Notch roles in development and diseases, while acknowledging the importance of combining classic and novel molecular tools and methods, bioinformatics workflows and applications, and different model organisms to fully understand the subject area. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Notch Signaling Research: Methods and Protocols is an ideal guide for researchers in the field, with techniques that could potentially be applied to study the wider world of other signaling pathways as well.

Editors and Affiliations

  • Department of Biology, Georgia Southern University, Statesboro, USA

    Dongyu Jia

Bibliographic Information

  • Book Title: Notch Signaling Research

  • Book Subtitle: Methods and Protocols

  • Editors: Dongyu Jia

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2201-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2200-1Published: 09 June 2022

  • Softcover ISBN: 978-1-0716-2203-2Published: 10 June 2023

  • eBook ISBN: 978-1-0716-2201-8Published: 08 June 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 298

  • Number of Illustrations: 11 b/w illustrations, 45 illustrations in colour

  • Topics: Cell Biology

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