Abstract
The clustered, regularly interspaced, short, palindromic repeat (CRISPR)-associated (CAS) nuclease Cas9 has been used in many organisms to generate specific mutations and transgene insertions. Here we describe our most up-to-date protocols using the S. pyogenes Cas9 in C. elegans that provides a convenient and effective approach for making heritable changes to the worm genome. We present several considerations when deciding which strategy best suits the needs of the experiment.
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Martin, C.J., Calarco, J.A. (2022). Approaches for CRISPR/Cas9 Genome Editing in C. elegans. In: Haspel, G., Hart, A.C. (eds) C. elegans. Methods in Molecular Biology, vol 2468. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2181-3_11
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DOI: https://doi.org/10.1007/978-1-0716-2181-3_11
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