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Glycan Microarrays pp 193–206Cite as

Glycan Microarrays Containing Synthetic Streptococcus pneumoniae CPS Fragments and Their Application to Vaccine Development

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2460))

Abstract

Streptococcus pneumoniae is the leading source of life-endangering diseases like pneumonia, septicemia, and meningitis, as well as a major cause of death in children under 5 years old in developing countries. At least 98 serotypes of S. pneumoniae can be distinguished based on their structurally distinct capsular polysaccharides (CPS). Currently available CPS-based pneumococcal vaccines contain serotypes most frequently associated with invasive pneumococcal diseases. The polysaccharides used in commercial conjugate-vaccines are isolated from bacteria cultures comprising many laborious and operationally challenging steps followed by depolymerization of long polysaccharides into small fragments and their conjugation to the carrier protein. The medicinal chemistry approach for glycoconjugate vaccine development offers an exciting alternative to CPS isolation for a broad range of different glycan antigens. Glycan arrays containing well-defined synthetic glycans of CPS fragments and repeating units are used as a platform for the high-throughput screening of various serum samples and identification of protective glycotopes for vaccine candidates.

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Acknowledgments

We thank the Max Planck Society and the German Research Foundation (SFB/TR 84 “Innate Immunity of the Lung,” C3, C6, C8) for the financial support. We also appreciate the help of ZIBI Graduate School and International Max Planck Research School for Infectious Diseases and Immunology program (IMPRS-IDI).

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Authors and Affiliations

  1. Department of Biomolecular Systems, Max-Planck-Institute of Colloids and Interfaces, Potsdam, Germany

    Paulina Kaplonek & Peter H. Seeberger

  2. Institute of Chemistry and Biochemistry, Freie Universitat Berlin, Berlin, Germany

    Paulina Kaplonek & Peter H. Seeberger

Authors
  1. Paulina Kaplonek
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  2. Peter H. Seeberger
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Corresponding author

Correspondence to Peter H. Seeberger .

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Editors and Affiliations

  1. Carbohydrate Signalling Group, Discipline of Microbiology School of Natural Sciences, National University of Ireland Galway, Galway, Ireland

    Michelle Kilcoyne

  2. Advanced Glycoscience Research Cluster, School of Natural Sciences, National University of Ireland Galway, Galway, Ireland

    Jared Q. Gerlach

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Kaplonek, P., Seeberger, P.H. (2022). Glycan Microarrays Containing Synthetic Streptococcus pneumoniae CPS Fragments and Their Application to Vaccine Development. In: Kilcoyne, M., Gerlach, J.Q. (eds) Glycan Microarrays. Methods in Molecular Biology, vol 2460. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2148-6_12

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  • DOI: https://doi.org/10.1007/978-1-0716-2148-6_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2147-9

  • Online ISBN: 978-1-0716-2148-6

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