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Immunohistochemical Detection of SARS-CoV-2 Antigens by Single and Multiple Immunohistochemistry

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SARS-CoV-2

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2452))

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Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection can be demonstrated in tissue sections by immunohistochemistry (IHC), which has the power to localize in bright field specific antigens in cells and tissues. The use of double or triple immunostains is capable of highlighting which cells are infected and/or the relationship of infected cell with other cells and tissue structures. In addition, immunoenzymatic multi-staining permits the simultaneous identification, localization, and enumeration of different cellular epitopes. Moreover, this method improves analytical precision, decreasing the time required for morphometric quantification, maximizing the information obtained from a single slide of paraffin-embedded tissue.

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Acknowledgments

SL and AV are supported by Fondazione Beretta (Brescia). Silvia Lonardi and Mattia Bugatti contributed equally to this work.

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Author notes

  1. Silvia Lonardi and Mattia Bugatti contributed equally to this work.

Authors and Affiliations

  1. Section of Pathology, Department of Molecular and Translational Medicine, University of Brescia, Spedali Civili di Brescia, Brescia, Italy

    Silvia Lonardi, Mattia Bugatti, Arianna Valzelli & Fabio Facchetti

Authors
  1. Silvia Lonardi
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  2. Mattia Bugatti
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  3. Arianna Valzelli
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  4. Fabio Facchetti
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Corresponding authors

Correspondence to Mattia Bugatti or Fabio Facchetti .

Editor information

Editors and Affiliations

  1. Department of Microbiology and Immunology; Infectious Disease TRD; BSL3 Core Facility, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore

    Justin Jang Hann Chu

  2. The University of British Columbia, Vancouver, BC, Canada

    Bintou Ahmadou Ahidjo

  3. BSL3 Core Facility, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore

    Chee Keng Mok

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Lonardi, S., Bugatti, M., Valzelli, A., Facchetti, F. (2022). Immunohistochemical Detection of SARS-CoV-2 Antigens by Single and Multiple Immunohistochemistry . In: Chu, J.J.H., Ahidjo, B.A., Mok, C.K. (eds) SARS-CoV-2. Methods in Molecular Biology, vol 2452. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2111-0_17

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  • DOI: https://doi.org/10.1007/978-1-0716-2111-0_17

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2110-3

  • Online ISBN: 978-1-0716-2111-0

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