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Purification and Proteomics Analysis of Phloem Tissues from Virus-Infected Plants

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Plant Virology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2400))

Abstract

The plant phloem vasculature is crucial for plant growth and development, and is essential for the systemic movement (SM) of plant viruses. Recent transcriptomic studies of the phloem during virus infection have shown the importance of this tissue, yet transcript levels do not provide definitive answers how virus–host interactions favour successful viral SM. Proteomic analyses have been used to identify host–virus protein interactions, uncovering a variety of ways by which viruses utilize host cellular machinery for completion of the viral infection cycle. Despite this new evidence through proteomics, very few phloem centric studies during viral infection have been performed. Here, we describe a protocol for the isolation of phloem tissues and proteins and the subsequent label-free quantitation (LFQ), for identification of proteomic alterations caused by viral infection.

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Acknowledgments

We would like to thank Dr. Justin Renaud (London Research and Development Centre, London, Ontario, Canada) for assistance with LC-MS/MS analysis. The protocol described here is relevant to the work supported by grants from Agriculture and Agri-Food Canada and the Natural Sciences and Engineering Research Council of Canada to A.W.

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Authors and Affiliations

  1. London Research and Development Centre, Agriculture and Agri-Food Canada, London, ON, Canada

    Aaron Simkovich & Aiming Wang

  2. Department of Biology, The University of Western Ontario, London, ON, Canada

    Aaron Simkovich & Susanne E. Kohalmi

Authors
  1. Aaron Simkovich
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  2. Susanne E. Kohalmi
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  3. Aiming Wang
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Corresponding author

Correspondence to Aiming Wang .

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Editors and Affiliations

  1. London Research and Development Centre, Agriculture and Agri-Food Canada, London, ON, Canada

    Aiming Wang

  2. State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, China

    Yi Li

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Simkovich, A., Kohalmi, S.E., Wang, A. (2022). Purification and Proteomics Analysis of Phloem Tissues from Virus-Infected Plants. In: Wang, A., Li, Y. (eds) Plant Virology . Methods in Molecular Biology, vol 2400. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1835-6_13

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  • DOI: https://doi.org/10.1007/978-1-0716-1835-6_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1834-9

  • Online ISBN: 978-1-0716-1835-6

  • eBook Packages: Springer Protocols

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