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Bead-Based Assays for Validating Proteomic Profiles in Body Fluids

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Protein Microarrays for Disease Analysis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2344))

Abstract

Protein biomarkers in biological fluids represent an important resource for improving the clinical management of diseases. Current proteomics technologies are capable of performing high-throughput and multiplex profiling in different types of fluids, often leading to the shortlisting of tens of candidate biomarkers per study. However, before reaching any clinical setting, these discoveries require thorough validation and an assay that would be suitable for routine analyses. In the path from biomarker discovery to validation, the performance of the assay implemented for the intended protein quantification is extremely critical toward achieving reliable and reproducible results. Development of robust sandwich immunoassays for individual candidates is challenging and labor and resource intensive, and multiplies when evaluating a panel of interesting candidates at the same time. Here we describe a versatile pipeline that facilitates the systematic and parallel development of multiple sandwich immunoassays using a bead-based technology.

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Acknowledgments

This work was supported by the funds from the Erling-Persson foundation for KTH Center for Precision Medicine (KCAP), Knut and Alice Wallenberg Foundation (KAW), KTH, and Science for Life Laboratory.

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Authors and Affiliations

  1. Science for Life Laboratory, Department of Protein Science, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, Stockholm, Sweden

    Annika Bendes, Matilda Dale, Cecilia Mattsson, Tea Dodig-Crnković, Maria Jesus Iglesias, Jochen M. Schwenk & Claudia Fredolini

  2. Department of Clinical Medicine, Faculty of Health Science, The Arctic University of Tromsö, Tromsö, Norway

    Maria Jesus Iglesias

Authors
  1. Annika Bendes
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  2. Matilda Dale
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  3. Cecilia Mattsson
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  4. Tea Dodig-Crnković
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  5. Maria Jesus Iglesias
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  6. Jochen M. Schwenk
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  7. Claudia Fredolini
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Corresponding author

Correspondence to Claudia Fredolini .

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Editors and Affiliations

  1. Functional Proteomics Unit, Instituto de Salud Carlos III, Madrid, Spain

    Rodrigo Barderas

  2. Biodesign Institute, Arizona State University, Tempe, AZ, USA

    Joshua LaBaer

  3. Proteomics Laboratory, Indian Institute of Technology Bombay, Mumbai, Maharashtra, India

    Sanjeeva Srivastava

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Bendes, A. et al. (2021). Bead-Based Assays for Validating Proteomic Profiles in Body Fluids. In: Barderas, R., LaBaer, J., Srivastava, S. (eds) Protein Microarrays for Disease Analysis. Methods in Molecular Biology, vol 2344. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1562-1_5

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  • DOI: https://doi.org/10.1007/978-1-0716-1562-1_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1561-4

  • Online ISBN: 978-1-0716-1562-1

  • eBook Packages: Springer Protocols

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