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Sepsis pp 191–205Cite as

Detection of Blood Cell Surface Biomarkers in Septic Mice

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2321))

Abstract

Sepsis arises when an infection induces a dysregulated immune response, resulting in organ damage. New methods are urgently needed to diagnose patients in the early stages of sepsis, and identify patients with a poor disease prognosis. One promising approach is to identify the rapid changes in cell surface antigens (biomarkers) that occur during sepsis, as a consequence of leukocyte mobilization and activation. This chapter describes the method for staining whole blood with fluorescently conjugated antibodies that detect cell surface biomarkers, and performing flow cytometry analysis to quantify biomarker-positive cells. Our protocol is designed to detect blood cell surface biomarkers in septic mice, but could also be applied to study potential biomarkers in blood obtained from human patients with sepsis and other medical conditions.

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Acknowledgments

This study was supported by a grant from the Society of Critical Care Medicine (SCCM) and startup funds from Texas Tech University Health Sciences Center at El Paso, awarded to Wendy Walker.

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Authors and Affiliations

  1. Center of Emphasis in Infectious Diseases, Department of Molecular and Translational Medicine, Paul L Foster School of Medicine, Texas Tech University Health Sciences Center at El Paso, El Paso, TX, USA

    Dinesh G. Goswami

  2. Department of Molecular and Translational Medicine, Texas Tech University Health Sciences Center at El Paso, El Paso, TX, USA

    Wendy E. Walker

Authors
  1. Dinesh G. Goswami
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  2. Wendy E. Walker
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Corresponding author

Correspondence to Wendy E. Walker .

Editor information

Editors and Affiliations

  1. Department of Molecular and Translational Medicine, Texas Tech University Health Sciences Center at El Paso, El Paso, TX, USA

    Wendy E. Walker

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Goswami, D.G., Walker, W.E. (2021). Detection of Blood Cell Surface Biomarkers in Septic Mice. In: Walker, W.E. (eds) Sepsis. Methods in Molecular Biology, vol 2321. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1488-4_17

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  • DOI: https://doi.org/10.1007/978-1-0716-1488-4_17

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1487-7

  • Online ISBN: 978-1-0716-1488-4

  • eBook Packages: Springer Protocols

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