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Cardiovascular Development

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Xu Peng

    1. Med. Physiology, Health Science Center, Texas A&M University, Bryan, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Warren E. Zimmer

    1. Med. Physiology, Health Science Center, Texas A&M University, Bryan, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2319)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Tissue Clearing and 3-D Visualization of Vasculature with the PEGASOS Method

    • Dian Jing, Yi Men, Hu Zhao
    Pages 1-13

  3. Preparation and Identification of Cardiac Myofibrils from Whole Heart Samples

    • Heidi A. Creed, Carl W. Tong
    Pages 15-24

  4. RNA-Sequencing and Transcriptome Analysis after Fluid Shear Stress Stimulation in Lymphatic Endothelial Cells

    • Hongjiang Si, Xu Peng
    Pages 25-30

  5. Preparation of Neonatal Rat Papillary Muscles for Contractile Studies

    • Steven Jokerst, Damir Nizmutdinov, Charley Edgar, April M. Kaspick, Carl W. Tong, David E. Dostal
    Pages 31-44

  6. In Vitro Study of Permeability in Lymphatic Endothelial Cells Responding to Histamine

    • Hongjiang Si
    Pages 45-49

  7. Optimizing the Differentiation of Cardiomyocytes from Human Induced Pluripotent-Derived Stem Cells

    • Melanie Gartz, Jennifer L. Strande
    Pages 51-60

  8. Analysis of Angiogenesis in Mouse Embryonic Dorsal Skin by Whole-Mount Fluorescent Staining

    • Jian Wang, Yuwei Dong, Yu Xi, Xu Peng
    Pages 61-67

  9. Isolation and Culture of Mouse Lymphatic Endothelial Cells from Lung Tissue

    • Philip E. Lapinski, Philip D. King
    Pages 69-75

  10. Laser-Induced Choroidal Neovascularization in Rats

    • Min Zhao, Wankun Xie, Travis W. Hein, Lih Kuo, Robert H. Rosa Jr
    Pages 77-85

  11. Detecting Three-Dimensional Vascular Networks in the Mouse Embryonic Hindbrain

    • Chenshen Huang, Liangjing Wu, Jian Wang, Binu Tharakan, Xu Peng
    Pages 87-92

  12. Visualizing Blood Vessel Development in Cultured Mouse Embryos Using Lightsheet Microscopy

    • Samantha J. Fredrickson, Tanner G. Hoog, Ryan S. Udan
    Pages 93-104

  13. Laser Capture Microdissection of Vascular Endothelial Cells from Frozen Heart Tissues

    • Tianhao Zhou, Jian Wang
    Pages 105-110

  14. Visualization of Retinal Blood Vessels

    • Wankun Xie, Min Zhao, Travis W. Hein, Lih Kuo, Robert H. Rosa Jr
    Pages 111-117

  15. Transient Transgenics: An Efficient Method to Identify Gene Regulatory Elements

    • Warren E. Zimmer, Robert J. Schwartz
    Pages 119-136

  16. Isolation of Lymphatic Muscle Cells (LMCs ) from Rat Mesentery

    • Xueyang Zhang, Sanjukta Chakraborty, Mariappan Muthuchamy, David C. Zawieja
    Pages 137-141

  17. Isolation of Adult Mouse Cardiomyocytes Using Langendorff Perfusion Apparatus

    • Yang Liu, David E. Dostal, Carl W. Tong
    Pages 143-152

  18. Analysis of Lymphatic Vessel Formation by Whole-Mount Immunofluorescence Staining

    • Jian Wang, Yuwei Dong, Mariappan Muthuchamy, David C. Zawieja, Xu Peng
    Pages 153-159

  19. Back Matter

    Pages 161-162
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Keywords

About this book

This second edition brings together a variety of method used in the field of cardiovascular development research. Chapters guide readers through protocols on lymphatic development, lymphatic staining techniques, lymphatic endothelial, tissue clearing techniques, and muscle cell isolation and culture. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls

Authoritative and practical, Cardiovascular Development: Methods and Protocols, Second Edition seeks to aid scientists in understanding new state-of-the-art techniques in the field of cardiovascular development research.

Editors and Affiliations

  • Med. Physiology, Health Science Center, Texas A&M University, Bryan, USA

    Xu Peng, Warren E. Zimmer

Bibliographic Information

  • Book Title: Cardiovascular Development

  • Book Subtitle: Methods and Protocols

  • Editors: Xu Peng, Warren E. Zimmer

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1480-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1479-2Published: 31 July 2021

  • Softcover ISBN: 978-1-0716-1482-2Published: 31 July 2022

  • eBook ISBN: 978-1-0716-1480-8Published: 30 July 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: X, 162

  • Number of Illustrations: 4 b/w illustrations, 33 illustrations in colour

  • Topics: Biomedicine, general, Cardiology

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