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Plastid Transformation in Physcomitrium (Physcomitrella) patens: An Update

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Chloroplast Biotechnology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2317))

Abstract

The moss Physcomitrium (Physcomitrella) patens performs efficient homologous recombination in both the nucleus and plastid enabling the study of individual gene function by generating precise inactivation or modification of genes. Polyethylene glycol (PEG)-mediated transformation of protoplasts is routinely used to study the nuclear gene function of P. patens. PEG-mediated protoplast transformation is also applied for plastid transformation of this moss. The efficiency of plastid transformation is quite reliable and one or two homoplasmic transplastomic lines are obtained in a plastid transformation experiment (5 × 105 protoplasts) by selection for spectinomycin resistance.

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Acknowledgments

The author would like to thank Ms. Chika Sugiura-Miyamoto for development of plastid transformation of P. patens. This work was supported by in part by a Grant-in-Aid from the Japan Society for the Promotion of Science (14340252).

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Authors and Affiliations

  1. Graduate School of Informatics, Nagoya University, Nagoya, Japan

    Mamoru Sugita

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  1. Mamoru Sugita
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Correspondence to Mamoru Sugita .

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Editors and Affiliations

  1. Waksman Institute of Microbiology, Rutgers University, Piscataway, NJ, USA

    Pal Maliga

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Sugita, M. (2021). Plastid Transformation in Physcomitrium (Physcomitrella) patens: An Update. In: Maliga, P. (eds) Chloroplast Biotechnology. Methods in Molecular Biology, vol 2317. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1472-3_19

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  • DOI: https://doi.org/10.1007/978-1-0716-1472-3_19

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1471-6

  • Online ISBN: 978-1-0716-1472-3

  • eBook Packages: Springer Protocols

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