Abstract
There are increasing evidence and growing interest in the relationship between protein aggregates/phase separation and various human diseases, especially neurodegenerative diseases. However, we do not entirely comprehend how aggregates generate or the clearance network of chaperones, proteasomes, ubiquitin ligases, and other factors interact with aggregates. Here, we describe chemically controllable systems compose with a genetically engineered cell and a small drug that enables us to rapidly induce protein aggregates’ formation by withdrawing the small molecule. This trigger does not activate global stress responses induced by stimuli, such as proteasome inhibitors or heat shock. This method can produce aggregates in a specific compartment and diverse experimental systems, including live animals.
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Acknowledgments
This work was supported by the NIH (GM073046 and P50 GM107615), the Ellison Medical Foundation (AG-SS-2573-10), and the Stanford Discovery Innovation Fund. Y.M. was supported by the Nakajima Foundation.
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Miyazaki, Y. (2021). AgDD System: A Chemical Controllable Protein Aggregates in Cells. In: Kojima, R. (eds) Mammalian Cell Engineering. Methods in Molecular Biology, vol 2312. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1441-9_16
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DOI: https://doi.org/10.1007/978-1-0716-1441-9_16
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