Abstract
Precipitation is a critical step to recover RNA of high purity. This chapter describes the principles of alcoholic precipitation as well as a standard, basic protocol with key advices to observe, but numerous variations on the theme are discussed. Indeed, several important parameters, such as the choice of salt, alcohol, or carrier, have to be considered to improve the efficiency of precipitation and the yield of RNA recovery.
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Bourguignon-Igel, V., Abel, Y., Rederstorff, M. (2021). RNA Precipitation. In: Rederstorff, M. (eds) Small Non-Coding RNAs. Methods in Molecular Biology, vol 2300. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1386-3_2
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DOI: https://doi.org/10.1007/978-1-0716-1386-3_2
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1385-6
Online ISBN: 978-1-0716-1386-3
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