Abstract
MicroRNAs (miRNAs) are a class of small noncoding single-stranded RNA molecules containing 18–22 nucleotides that play an important role in the regulation of gene expression at the post-transcriptional and translational levels. Loss-of-function studies are the fundamental strategy to examine miRNA function and target genes in cellular and molecular biology. Traditional methods for miRNA loss-of-function studies include miRNA-specific antisense inhibitors, miRNA sponges, and genetic knockout. However, efficiency, specificity, and stability of these methods are not adequate. Our study suggests that CRISPR/Cas9 is an economic, convenient, and innovative strategy with high efficiency, specificity, and stability for the modulation of miRNA expression. Herein, we describe a detailed protocol for knocking out miRNA genes in vitro and in vivo with the CRISPR/Cas9 system.
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Yi, B., Larter, K., Xi, Y. (2021). CRISPR/Cas9 System to Knockdown MicroRNA In Vitro and In Vivo. In: Rederstorff, M. (eds) Small Non-Coding RNAs. Methods in Molecular Biology, vol 2300. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1386-3_13
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DOI: https://doi.org/10.1007/978-1-0716-1386-3_13
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1385-6
Online ISBN: 978-1-0716-1386-3
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