Skip to main content
SpringerLink
Log in

Rab GTPases

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Guangpu Li

    1. Department of Biochemistry and Molecular Biology, Peggy and Charles Stephenson Cancer Center, University of Oklahoma Health Sciences Center, Oklahoma City, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Nava Segev

    1. Department of Biochemistry and Molecular Genetics, College of Medicine, University of Illinois at Chicago, Chicago, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2293)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 149.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (21 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Newer Methods Drive Recent Insights into Rab GTPase Biology: An Overview

    • Guangpu Li, Nava Segev
    Pages 1-18

  3. Rab29 Fast Exchange Mutants : Characterization of a Challenging Rab GTPase

    • Rachel C. Gomez, Edmundo G. Vides, Suzanne R. Pfeffer
    Pages 19-25

  4. High-Throughput Assay for Profiling the Substrate Specificity of Rab GTPase-Activating Proteins

    • Ashwini K. Mishra, David G. Lambright
    Pages 27-43

  5. Detecting Endogenous Rab8 Activation

    • Samuel J. Tong, Richard M. Lucas, Zhijian Xiao, Lin Luo, Jennifer L. Stow
    Pages 45-56

  6. Testing the Phenotypic Effects of a Rab Chimera that Resolves Exchange Factor Specificity from Effector Specificity

    • Hua Yuan, Peter Novick
    Pages 57-67

  7. Profiling Structural Alterations During Rab5 Nucleotide Exchange by HDX-MS

    • Janelle Lauer, Marino Zerial
    Pages 69-89

  8. CLEM Characterization of Rab8 and Associated Membrane Trafficking Regulators at Primary Cilium Structures

    • Quanlong Lu, Christopher J. Westlake
    Pages 91-103

  9. Imaging of Spatial Cycling of Rab GTPase in the Cell

    • Fu Li, Yao-Wen Wu
    Pages 105-115

  10. Deconvolution of Multiple Rab Binding Domains Using the Batch Yeast 2-Hybrid Method DEEPN

    • Tabitha A. Peterson, Robert C. Piper
    Pages 117-141

  11. Determination of the Rab27–Effector Binding Affinity Using a High-Throughput FRET-Based Assay

    • Raghdan Z. Al-Saad, Ian Kerr, Alistair N. Hume
    Pages 143-162

  12. Methods to Study the Unique SOCS Box Domain of the Rab40 Small GTPase Subfamily

    • Emily D. Duncan, Ezra Lencer, Erik Linklater, Rytis Prekeris
    Pages 163-179

  13. Using GBP Nanotrap to Restore Autophagy in the Rab5 / Vps21 Mutant by Forcing Snf7 and Atg17 Interaction

    • Mengzhu Zhao, Yongheng Liang
    Pages 181-188

  14. Establishing Regulation of a Dynamic Process by Ypt/Rab GTPases : A Case for Cisternal Progression

    • Jane J. Kim, Zanna Lipatova, Nava Segev
    Pages 189-199

  15. Methods for Assessing the Regulation of a Kinase by the Rab GTPase Ypt1

    • Juan Wang, Shensen Wang, Susan Ferro-Novick
    Pages 201-211

  16. Qualitative and Quantitative Assessment of the Role of Endocytic Regulatory and/or Rab Proteins on Mitochondrial Fusion and Fission

    • Trey Farmer, Steve Caplan
    Pages 213-227

  17. Characterization of the Role of Rab18 in Mediating LD –ER Contact and LD Growth

    • Dijin Xu, Peng Li, Li Xu
    Pages 229-241

  18. Methods for Establishing Rab Knockout MDCK Cells

    • Riko Kinoshita, Yuta Homma, Mitsunori Fukuda
    Pages 243-256

  19. Generating Rab6 Conditional Knockout Mice

    • Sabine Bardin, Bruno Goud
    Pages 257-263

  20. Use of Immunohistochemistry to Determine Expression of Rab5 Subfamily of GTPases in Mature and Developmental Brains

    • Kwok-Ling Kam, Paige Parrack, Marcellus Banworth, Sheeja Aravindan, Guangpu Li, Kar-Ming Fung
    Pages 265-271
Back to top

Keywords

About this book

This second edition volume expands on the previous edition with a discussion of new research and discoveries in the Rab field. Chapters in this book cover topics such as new information on Rab regulation and localization; interaction; function; and diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Cutting-edge and comprehensive, Rab GTPases: Methods and Protocols, Second Edition is a valuable resource for scientists working in the fields of Rab and other small GTPases, and beyond.


Editors and Affiliations

  • Department of Biochemistry and Molecular Biology, Peggy and Charles Stephenson Cancer Center, University of Oklahoma Health Sciences Center, Oklahoma City, USA

    Guangpu Li

  • Department of Biochemistry and Molecular Genetics, College of Medicine, University of Illinois at Chicago, Chicago, USA

    Nava Segev

Bibliographic Information

  • Book Title: Rab GTPases

  • Book Subtitle: Methods and Protocols

  • Editors: Guangpu Li, Nava Segev

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1346-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1345-0Published: 29 August 2021

  • Softcover ISBN: 978-1-0716-1348-1Published: 30 August 2022

  • eBook ISBN: 978-1-0716-1346-7Published: 28 August 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XII, 310

  • Topics: Biochemistry, general

Publish with us

Policies and ethics

Back to top

Search

Navigation