Abstract
Fluorescence (FL)-guided detection of cancer is one of the most promising approaches to achieve intraoperative assessment of surgical margins. Enzymes, such as aminopeptidase, carboxypeptidase, and glycosidase, whose activities are increased in cancer, have attracted great interest as imaging targets for rapid and sensitive visualization of cancerous tissues with fluorescent probes. Activatable probes, which are initially nonfluorescent but become strongly fluorescent upon rapid one-step cleavage of their substrate moiety by the target enzyme, are especially promising for practical clinical application during surgical or endoscopic procedures due to the highly amplified FL change generated by enzyme-catalyzed turnover at lesion sites. Here, we describe robust protocols for using activatable fluorescent probes targeting cancer-associated enzyme activities to visualize cultured cancer cells, metastatic cancer in a mouse model, and cancerous lesions in surgical specimens from patients.
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Acknowledgments
This work was supported in part by AMED under grant number JP19gm0710008 (to Y.U.), by JST/PRESTO grant JPMJPR14F8 (to M.K.), by MEXT/JSPS KAKENHI grants JP16H02606, JP26111012, and JP19H05632 (to Y.U.) and JP15H05951 “Resonance Bio”, JP19H02826, and JP19K22242 (to M.K.), by JSPS Core-to-Core Program (grant number: JPJSCCA20170007), A. Advanced Research Networks, by Japan Foundation for Applied Enzymology (to M.K.), and The Naito Foundation (to M.K.), as well as a stipend from the Graduate Program for Leaders in Life Innovation (GPLLI) (to K.F.), World-leading Innovative Graduate Study Program for Life Science and Technology (WINGS-LST) (to K.F.), Masason Foundation (to K.F.) and a JSPS stipend (to K.F.).
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Fujita, K., Kamiya, M., Urano, Y. (2021). Rapid and Sensitive Detection of Cancer Cells with Activatable Fluorescent Probes for Enzyme Activity. In: Kim, SB. (eds) Live Cell Imaging. Methods in Molecular Biology, vol 2274. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1258-3_17
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DOI: https://doi.org/10.1007/978-1-0716-1258-3_17
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