Abstract
Recent methodological advances in both gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS) have provided a deep understanding of metabolic regulation occurring in plant cells. The application of these techniques to agricultural systems is, however, subject to more complex interactions. Here we summarize a step-by-step modern metabolomics methodology that generates metabolome data toward the implementation of metabolomics in crop breeding. We describe a metabolic workflow, and provide guidelines for handling large sample numbers for the specific purpose of metabolic quantitative trait loci approaches.
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Acknowledgments
SA and ARF acknowledge funding of the PlantaSYST project by the European Union’s Horizon 2020 Research and Innovation Programme (SGA‐CSA no. 664621 and no. 739582 under FPA no. 664620).
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Alseekh, S., Fernie, A.R. (2021). Using Metabolomics to Assist Plant Breeding. In: Tripodi, P. (eds) Crop Breeding. Methods in Molecular Biology, vol 2264. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1201-9_3
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