Abstract
Identifying the proteins that associate with RAS oncoproteins has great potential, not only to elucidate how these mutant proteins are regulated and signal but also to identify potential therapeutic targets. Here we describe a detailed protocol to employ proximity labeling by the BioID methodology, which has the advantage of capturing weak or transient interactions, to identify in an unbiased manner those proteins within the immediate vicinity of oncogenic RAS proteins.
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Acknowledgments
This work was supported by R01CA123031 and P01CA203657 (C.M.C.) and by a Pancreatic Cancer Action Network-NCI Fredrick National Laboratory for Cancer Research KRAS Fellowship and K99CA248495 (H.A.).
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Adhikari, H., Counter, C.M. (2021). Using BioID to Characterize the RAS Interactome. In: Rubio, I., Prior, I. (eds) Ras Activity and Signaling. Methods in Molecular Biology, vol 2262. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1190-6_16
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DOI: https://doi.org/10.1007/978-1-0716-1190-6_16
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1189-0
Online ISBN: 978-1-0716-1190-6
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