Abstract
The CRISPR/Cas9 system has been widely used as an efficient genome-editing tool for studying physiological functions of long noncoding RNAs (lncRNAs). In this chapter, we describe the experimental procedures for using the CRISPR/Cas9 system to genetically modify a long noncoding RNA in vivo through the targeted disruption and knockin approaches.
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Acknowledgments
Dr. Xi Cheng acknowledges funding support of the Dean’s Postdoctoral to Faculty Fellowship from University of Toledo College of Medicine and Life Sciences and the P30 Core Center Pilot Grant from NIDA Center of Excellence in Omics, Systems Genetics, and the Addictome. Dr. Bina Joe acknowledges grant support from the National Heart, Lung, and Blood Institute.
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Cheng, X., Peters, S.T., Pruett-Miller, S.M., Saunders, T.L., Joe, B. (2021). In Vivo CRISPR/Cas9-Based Targeted Disruption and Knockin of a Long Noncoding RNA. In: Cao, H. (eds) Functional Analysis of Long Non-Coding RNAs. Methods in Molecular Biology, vol 2254. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1158-6_19
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DOI: https://doi.org/10.1007/978-1-0716-1158-6_19
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1157-9
Online ISBN: 978-1-0716-1158-6
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