Abstract
The physiological, pathological, and regenerative roles of pericytes as microvascular mural cells and multipotent precursors have gained significant attention. The capacity to prospectively purify pericytes from multiple organs enables the investigation of their tissue-specific regenerative capabilities. Here, we describe the application of purified human pericytes for cardiac regeneration post-infarct in an immunodeficient mouse model. This protocol includes experimental details of pericyte isolation from both human skeletal and cardiac muscle, an immunodeficient mouse model of acute myocardial infarction, and xenogeneic pericyte transplantation.
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Acknowledgment
J.E.B. was supported by a British Heart Foundation Centre of Research Excellence doctoral training award (RE/08/001/23904). W.C. was supported by the NIH Ruth L. Kirschstein NRSA postdoctoral fellowship (5T32HL007208). The authors declare no conflict of interest.
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Baily, J.E., Chen, W.C.W. (2021). Transplantation of Purified Human Pericytes for Myocardial Repair in SCID-hu Mice. In: Péault, B.M. (eds) Pericytes. Methods in Molecular Biology, vol 2235. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1056-5_2
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DOI: https://doi.org/10.1007/978-1-0716-1056-5_2
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-1056-5
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