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Anti-C1-Inhibitor Autoantibody Detection by ELISA

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The Complement System

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2227))

Abstract

Enzyme-linked immunosorbent assay (ELISA) is a quantitative analytical method used to measure the concentration of molecules in biological fluids through antigen-antibody reactions. Here we describe the measurement of anti-C1-inhibitor autoantibodies by an indirect ELISA. In this method patients’ sera are incubated in a microplate coated with plasma derived C1-inhibitor.

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Suffritti, C., Caccia, S., Berra, S., Parolin, D., Cicardi, M. (2021). Anti-C1-Inhibitor Autoantibody Detection by ELISA. In: Roumenina, L.T. (eds) The Complement System. Methods in Molecular Biology, vol 2227. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1016-9_11

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  • DOI: https://doi.org/10.1007/978-1-0716-1016-9_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1015-2

  • Online ISBN: 978-1-0716-1016-9

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