Skip to main content
SpringerLink
Log in

Molecular Typing of Salmonella by Pulsed-Field Gel Electrophoresis

  • Protocol
  • First Online:
Salmonella

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2182))

  • 965 Accesses

Abstract

Salmonella is recognized as a major human foodborne pathogen and threat to public health world widely. It is important to carry out epidemiological investigations to determine the primary sources of bacterial contamination. Pulsed-field gel electrophoresis (PFGE) is an important method of the molecular typing, and play an important role in tracking the sources of infection and epidemic control. The PFGE is currently considered as “gold standard” of molecular typing methods for bacterial foodborne pathogen. Here, we describe the PFGE protocol to type the Salmonella from pork.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 179.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Luna S, Taylor M, Galanis E et al (2018) Outbreak of Salmonella chailey infections linked to precut coconut pieces-United States and Canada, 2017. MMWR Morb Mortal Wkly Rep 67:1098–1100

    Article  Google Scholar 

  2. Xu C, Ren X, Feng Z et al (2017) Phenotypic characteristics and genetic diversity of Salmonella enterica serotype Derby isolated from human patients and foods of animal origin. Foodborne Pathog Dis 14:593–599

    Article  CAS  Google Scholar 

  3. Majowicz SE, Musto J, Scallan E (2010) The global burden of nontyphoidal Salmonella gastroenteritis. Clin Infect Dis 50:882–889

    Article  Google Scholar 

  4. Foley SL, Lynne AM, Nayak R (2009) Molecular typing methodologies for microbial source tracking and epidemiological investigations of Gram-negative bacterial foodborne pathogens. Infect Genet Evol 9:430–440

    Article  CAS  Google Scholar 

  5. Foley SL, Zhao S, Walker RD (2007) Comparison of molecular typing methods for the differentiation of Salmonella foodborne pathogens. Foodborne Pathog Dis 4:253–276

    Article  CAS  Google Scholar 

  6. Swoveland JL, Stewart LK, Eckmann MK et al (2019) Laboratory review of foodborne disease investigations in Washington State 2007–2017. Foodborne Pathog Dis 16:513–523

    Article  Google Scholar 

  7. Timme RE, Strain E, Baugher JD et al (2019) Phylogenomic pipeline validation for foodborne pathogen disease surveillance. J Clin Microbiol 57. pii: e01816-18

    Google Scholar 

  8. Neoh HM, Tan XE, Sapri HF et al (2019) Pulsed-field gel electrophoresis (PFGE): a review of the “gold standard” for bacteria typing and current alternatives. Infect Genet Evol 74:103935

    Article  CAS  Google Scholar 

  9. Olive DM, Bean P (1999) Principles and applications of methods for DNA-based typing of microbial organisms. J Clin Microbiol 37:1661–1669

    Article  CAS  Google Scholar 

  10. Winokur PL (2003) Molecular epidemiological techniques for Salmonella strain discrimination. Front Biosci 8:c14–c24

    Article  CAS  Google Scholar 

  11. Ribot EM, Fair MA, Gautom R et al (2006) Standardization of pulsed-field gel electrophoresis protocols for the subtyping of Escherichia coli O157:H7, Salmonella, and Shigella for PulseNet. Foodborne Pathog Dis 3:59–67

    Article  CAS  Google Scholar 

  12. Wattiau P, Boland C, Bertrand S (2011) Methodologies for Salmonella enterica subsp. enterica subtyping: gold standards and alternatives. Appl Environ Microbiol 77:7877–7885

    Article  CAS  Google Scholar 

  13. Cai Y, Tao J, Jiao Y et al (2016) Phenotypic characteristics and genotypic correlation between Salmonella isolates from a slaughterhouse and retail markets in Yangzhou, China. Int J Food Microbiol 222:56–64

    Article  CAS  Google Scholar 

  14. Li Y, Cai Y, Tao J et al (2016) Salmonella isolated from the slaughterhouses and correlation with pork contamination in free market. Food Control 59:591–600

    Article  Google Scholar 

  15. Zhou Z, Li J, Zheng H et al (2017) Diversity of Salmonella isolates and their distribution in a pig slaughterhouse in Huaian, China. Food Control 78:238–246

    Article  Google Scholar 

  16. Fois F, Piras F, Torpdahl M et al (2017) Occurrence, characterization, and antimicrobial susceptibility of Salmonella enterica in slaughtered pigs in Sardinia. J Food Sci 82:969–976

    Article  CAS  Google Scholar 

  17. Grimont PAD, Weill FX (2007) Antigenic formulae of the Salmonella serovars. In: World health organization collaborating center for reference and research on Salmonella, 9th edn. Institut Pasteur, Paris

    Google Scholar 

Download references

Acknowledgements

This work was supported by the National Key Research and Development Program Special Project [2016YFD0501607, 2018YFD0500502], the Special Fund for Agroscientific Research in the Public Interest [201403054], the Yangzhou University Science and Technology Innovation Team, and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

Author information

Authors and Affiliations

  1. Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, Jiangsu, China

    Xilong Kang, Chuang Meng & Zhiming Pan

  2. Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu, China

    Xilong Kang, Chuang Meng & Zhiming Pan

  3. Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, MOA, Yangzhou University, Yangzhou, Jiangsu, China

    Xilong Kang, Chuang Meng & Zhiming Pan

  4. Joint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of Education, Yangzhou, Jiangsu, China

    Xilong Kang, Chuang Meng & Zhiming Pan

Authors
  1. Xilong Kang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Chuang Meng
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zhiming Pan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zhiming Pan .

Editor information

Editors and Affiliations

  1. Department of Veterinary Pathobiology, University of Missouri-Columbia, Columbia, MO, USA

    Heide Schatten

Rights and permissions

Reprints and permissions

Copyright information

© 2021 Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Kang, X., Meng, C., Pan, Z. (2021). Molecular Typing of Salmonella by Pulsed-Field Gel Electrophoresis. In: Schatten, H. (eds) Salmonella. Methods in Molecular Biology, vol 2182. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0791-6_17

Download citation

  • DOI: https://doi.org/10.1007/978-1-0716-0791-6_17

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0790-9

  • Online ISBN: 978-1-0716-0791-6

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation