Abstract
The epithelial to mesenchymal transition (EMT) is an enticingly simple mechanism that converts stationary epithelial cells into migratory mesenchymal cells. EMT is meant to provide a unified explanation for phenomena as complex as gastrulation and metastasis. However, cell movements turn out to be diverse, and many are collective. Cells commonly migrate in clusters, strands, sheets, elongating tubes, or in fluid-like masses. Moreover, plenty of cells move without activating the EMT program. Here I propose that EMT can be understood as one of many types of transitions in a broader landscape—or phase space—of cell morphologies and behaviors. Throughout biology, and at multiple scales, complexity arises from the combinatorial deployment of simple, modular components. I propose that diversity of cell shapes and behaviors similarly arises from combinatorial use of modular biomechanical properties.
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References
Thiery JP et al (2009) Epithelial-mesenchymal transitions in development and disease. Cell 139:871–890
Lim J, Thiery JP (2012) Epithelial-mesenchymal transitions: insights from development. Development 139:3471–3486
Lambert AW et al (2017) Emerging biological principles of metastasis. Cell 168:670–691
Nieto MA et al (2016) EMT: 2016. Cell 166:21–45
Friedl P, Gilmour D (2009) Collective cell migration in morphogenesis, regeneration and cancer. Nat Rev Mol Cell Biol 10:445–457
Derynck R, Weinberg RA (2019) EMT and cancer: more than meets the eye. Dev Cell 49:313–316
Shamir ER et al (2014) Twist1-induced dissemination preserves epithelial identity and requires E-cadherin. J Cell Biol 204:839–856
Cheung KJ et al (2016) Polyclonal breast cancer metastases arise from collective dissemination of keratin 14-expressing tumor cell clusters. Proc Natl Acad Sci U S A 113:E854–E863
Aceto N et al (2014) Circulating tumor cell clusters are oligoclonal precursors of breast cancer metastasis. Cell 158:1110–1122
Cheung KJ, Ewald AJ (2016) A collective route to metastasis: seeding by tumor cell clusters. Science 352:167–169
Kröger C et al (2019) Acquisition of a hybrid E/M state is essential for tumorigenicity of basal breast cancer cells. Proc Natl Acad Sci U S A 116:7353–7362
Thiery JP, Chopin D (1999) Epithelial cell plasticity in development and tumor progression. Cancer Metastasis Rev 18(1):31–42
Beerling E et al (2016) Plasticity between epithelial and mesenchymal states unlinks EMT from metastasis-enhancing stem cell capacity. Cell Rep 14:2281–2288
Friedl P, Wolf K (2010) Plasticity of cell migration: a multiscale tuning model. J Cell Biol 188:11–19
Montell DJ et al (2012) Group choreography: mechanisms orchestrating the collective movement of border cells. Nat Rev Mol Cell Biol 13:631–645
Montell DJ (2008) Morphogenetic cell movements: diversity from modular mechanical properties. Science 322:1502–1505
Hannezo E et al (2014) Theory of epithelial sheet morphology in three dimensions. Proc Natl Acad Sci U S A 111:27–32
DiMilla PA et al (1991) Mathematical model for the effects of adhesion and mechanics on cell migration speed [published erratum appears in Biophys J 1991 Oct;60(4):983]. Biophys J 60(1):15–37
Yamazaki D et al (2009) Involvement of Rac and Rho signaling in cancer cell motility in 3D substrates. Oncogene 28:1570–1583
Panková K et al (2010) The molecular mechanisms of transition between mesenchymal and amoeboid invasiveness in tumor cells. Cell Mol Life Sci 67:63–71
Mishra AK et al (2019) Coordination of protrusion dynamics within and between collectively migrating border cells by myosin II. Mol Biol Cell 30(19):2490–2502. https://doi.org/10.1091/mbc.E19-02-0124
Lämmermann T, Sixt M (2009) Mechanical modes of “amoeboid” cell migration. Curr Opin Cell Biol 21:636–644
Murrell M et al (2015) Forcing cells into shape: the mechanics of actomyosin contractility. Nat Rev Mol Cell Biol 16:486–498
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Montell, D.J. (2021). EMT, One of Many Morphological Transitions in Cellular Phase Space. In: Campbell, K., Theveneau, E. (eds) The Epithelial-to Mesenchymal Transition. Methods in Molecular Biology, vol 2179. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0779-4_3
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DOI: https://doi.org/10.1007/978-1-0716-0779-4_3
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