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The Epithelial-to Mesenchymal Transition

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Kyra Campbell

    1. Department of Biomedical Science and Bateson Centre, University of Sheffield, Sheffield, UK

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  2. Eric Theveneau

    1. Centre de Biologie du Développement (CBD) Centre de Biologie Intégrative (CBI), Université de Toulouse, Toulouse, France

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2179)

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Table of contents (29 protocols)

  1. Front Matter

    Pages i-xv
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  2. Perspectives on the Past and Future of EMT Research

    1. Front Matter

      Pages 1-1
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    2. A Spectrum of Cell States During the Epithelial-to-Mesenchymal Transition

      • Erica J. Hutchins, Marianne E. Bronner
      Pages 3-6

    3. Perspective on Epithelial-Mesenchymal Transitions in Embryos

      • David R. McClay
      Pages 7-12

    4. EMT, One of Many Morphological Transitions in Cellular Phase Space

      • Denise J. Montell
      Pages 13-18

    5. Are You Interested or Afraid of Working on EMT?

      • M. Angela Nieto
      Pages 19-28

    6. Partial EMT/MET: An Army of One

      • Sofiane Hamidi, Hiroki Nagai, Guojun Sheng
      Pages 29-33

    7. EMT: An Update

      • Jean Paul Thiery
      Pages 35-39

  3. Review on Mesenchymal-to-Epithelial Transitions

    1. Front Matter

      Pages 41-41
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    2. Mesenchymal-to-Epithelial Transitions in Development and Cancer

      • John-Poul Ng-Blichfeldt, Katja Röper
      Pages 43-62

  4. Methods to Study EMT in In Vivo Systems

    1. Front Matter

      Pages 63-63
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    2. Live Imaging of Epithelial-Mesenchymal Transition in Mesoderm Cells of Gastrulating Drosophila Embryos

      • Lingkun Gu, Mo Weng
      Pages 65-77

    3. Zebrafish Neural Crest: Lessons and Tools to Study In Vivo Cell Migration

      • Zain Alhashem, Macarena Alvarez-Garcillan Portillo, Mint Ravinand Htun, Anton Gauert, Luis Briones Montecinos, Steffen Härtel et al.
      Pages 79-106

    4. Live Imaging of the Neural Crest Cell Epithelial-to-Mesenchymal Transition in the Chick Embryo

      • Mary Cathleen McKinney, Paul M. Kulesa
      Pages 107-114

    5. Exploiting Drosophila melanogaster Wing Imaginal Disc Eversion to Screen for New EMT Effectors

      • Sofia Golenkina, Rosemary Manhire-Heath, Michael J. Murray
      Pages 115-134

    6. Visualizing Mouse Embryo Gastrulation Epithelial-Mesenchymal Transition Through Single Cell Labeling Followed by Ex Vivo Whole Embryo Live Imaging

      • Wallis Nahaboo, Bechara Saykali, Navrita Mathiah, Isabelle Migeotte
      Pages 135-144

    7. Fluorescence Recovery After Photobleaching to Study the Dynamics of Membrane-Bound Proteins In Vivo Using the Drosophila Embryo

      • Joshua Greig, Natalia A. Bulgakova
      Pages 145-159

    8. Methods to Generate and Assay for Distinct Stages of Cancer Metastasis in Adult Drosophila melanogaster

      • Jamie Adams, Andreu Casali, Kyra Campbell
      Pages 161-170

  5. In Vivo Imaging of MET

    1. Front Matter

      Pages 171-171
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    2. 4D Live Imaging and Analysis of Chick Embryo Somites

      • Gi Fay Mok, James McColl, Andrea Münsterberg
      Pages 173-181
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Keywords

About this book

This volume details a comprehensive range of methods for imaging  epithelial-to-mesechymal transition (EMT)/MET in in vivo systems, and methods to leverage these systems to dissect the underlying mechanisms. Chapters guide readers through studying different features of epithelial-mesenchymal plasticity, past and future research of the EMT, in vivo systems, and in vivo imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, The Epithelial-to Mesenchymal Transition: Methods and Protocols aims to provide methods in EMT will help to unite and drive research in this exciting field forwards.


Editors and Affiliations

  • Department of Biomedical Science and Bateson Centre, University of Sheffield, Sheffield, UK

    Kyra Campbell

  • Centre de Biologie du Développement (CBD) Centre de Biologie Intégrative (CBI), Université de Toulouse, Toulouse, France

    Eric Theveneau

Bibliographic Information

  • Book Title: The Epithelial-to Mesenchymal Transition

  • Book Subtitle: Methods and Protocols

  • Editors: Kyra Campbell, Eric Theveneau

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0779-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-0778-7Published: 17 September 2020

  • Softcover ISBN: 978-1-0716-0781-7Published: 18 September 2021

  • eBook ISBN: 978-1-0716-0779-4Published: 16 September 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 429

  • Number of Illustrations: 6 b/w illustrations, 79 illustrations in colour

  • Topics: Cell Biology

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