Abstract
We recently described the generation of a novel mouse strain that efficiently and readily engrafts human primary hepatocytes to create liver xenografts (Borel et al., Mol Ther, 25: 2477–89, 2017). A transgenic mouse strain expressing a human PiZ allele for the SerpinA1 gene was crossed with the NOD-SCID-gamma chain knockout (NSG) strain to create a recipient strain (PiZ-NSG) for human hepatocyte xenotransplantation. In this chapter we provide a description of the methods to achieve these liver xenografts in the PiZ-NSG mouse.
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Reference
Borel F, Tang Q, Gernoux G, Greer C, Wang Z, Barzel A et al (2017) Survival advantage of both human hepatocyte xenografts and genome-edited hepatocytes for treatment of alpha-1 antitrypsin deficiency. Mol Ther 25(11):2477–2489
Acknowledgments
The mouse liver diagram in Fig. 1 was created by Mr. Hong Ma, Gene Therapy Center University of Massachusetts Medical School.
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Tang, Q., Gernoux, G., Cheng, Y., Flotte, T., Mueller, C. (2020). Engraftment of Human Hepatocytes in the PiZ-NSG Mouse Model. In: Aouadi, M., Azzimato, V. (eds) Kupffer Cells. Methods in Molecular Biology, vol 2164. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0704-6_9
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DOI: https://doi.org/10.1007/978-1-0716-0704-6_9
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-0703-9
Online ISBN: 978-1-0716-0704-6
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