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High-Voltage Paper Electrophoresis (HVPE)

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The Plant Cell Wall

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2149))

Abstract

HVPE is an excellent and often overlooked method for obtaining objective and meaningful information about cell-wall “building blocks” and their metabolic precursors. It provides not only a means of analysis of known compounds but also an insight into the charge and/or mass of any unfamiliar compounds that may be encountered. It can be used preparatively or analytically. It can achieve either “class separations” (e.g., delivering all hexose monophosphates into a single pool) or the resolution of different compounds within a given class (e.g., ADP-Glc from UDP-Glc; or GlcA from GalA).

All information from HVPE about charge and mass can be obtained on minute traces of analytes, especially those that have been radiolabeled, for example by in-vivo feeding of a 3H- or 14C-labeled precursor. HVPE does not usually damage the substance under investigation (unless staining is used), so samples of interest can be eluted intact from the paper ready for further analysis. Although HVPE is a technique that has been available for several decades, recently it has tended to be sidelined, possible because the apparatus is not widely available. Interested scientists are invited to contact the author about the possibility of accessing the Edinburgh apparatus.

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Acknowledgments

I thank numerous past and present colleagues and students for electrophoretic data and experience presented in this chapter and the UK BBSRC for financial support.

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Authors and Affiliations

  1. The Edinburgh Cell Wall Group, Institute of Molecular Plant Sciences, School of Biological Sciences, The University of Edinburgh, Edinburgh, UK

    Stephen C. Fry

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  1. Stephen C. Fry
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Correspondence to Stephen C. Fry .

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Editors and Affiliations

  1. Botany and Plant Science School of Natural Sciences, Ryan Institute for Environmental Marine and Energy Research School of Natural Sciences, The National University of Ireland Galway, Galway, Ireland

    Zoë A. Popper

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Fry, S.C. (2020). High-Voltage Paper Electrophoresis (HVPE). In: Popper, Z. (eds) The Plant Cell Wall. Methods in Molecular Biology, vol 2149. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0621-6_1

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  • DOI: https://doi.org/10.1007/978-1-0716-0621-6_1

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0619-3

  • Online ISBN: 978-1-0716-0621-6

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