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In Vivo Analysis of Glial Immune Responses to Axon Degeneration in Drosophila melanogaster

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Axon Degeneration

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2143))

Abstract

Axon degeneration elicits a range of immune responses from local glial cells, including striking changes in glial gene expression, morphology, and phagocytic activity. Here, we describe a detailed set of protocols to assess discrete components of the glial reaction to axotomy in the adult nervous system of Drosophila melanogaster. These methods allow one to visualize and quantify transcriptional, morphological, and functional responses of glia to degenerating axons in a model system that is highly amenable to genetic manipulation.

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Acknowledgments

We thank Maria Purice, Petra Richer, Derek Musashe, and Lilly Winfree for outstanding technical and intellectual contributions to this work. We thank Marc Freeman, Dirk Bohmann, Bloomington Drosophila Stock Center at Indiana University, and the Developmental Studies Hybridoma Bank at the University of Iowa for flies and antibodies. Funding: NIH R21 NS084112 (M.A.L. and S.D.S.), NIH R21 NS107771 (M.A.L.), NSF1557975 (S.D.S.), and the Ken and Ginger Harrison Term Professor Award (M.A.L.).

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Authors and Affiliations

  1. Jungers Center for Neurosciences Research, Department of Neurology, Oregon Health and Science University, Portland, OR, USA

    Mary A. Logan & Sean D. Speese

Authors
  1. Mary A. Logan
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  2. Sean D. Speese
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Corresponding author

Correspondence to Mary A. Logan .

Editor information

Editors and Affiliations

  1. Department of Biochemistry, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, NY, USA

    Elisabetta Babetto

1 5. Electronic Supplementary Material

Netwell construction (MP4 1,087,572 kb)

Maxillary and Antennal Ablation (MP4 115,683 kb)

Adult VNC injury (MP4 79,269 kb)

Pulling adult Drosophila Heads (MP4 56,147 kb)

Adult Brain Dissection (MP4 74,073 kb)

Adult VNC Dissection (MP4 181,843 kb)

Revised Mounting adult CNS (MP4 312,061 kb)

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Logan, M.A., Speese, S.D. (2020). In Vivo Analysis of Glial Immune Responses to Axon Degeneration in Drosophila melanogaster. In: Babetto, E. (eds) Axon Degeneration. Methods in Molecular Biology, vol 2143. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0585-1_24

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  • DOI: https://doi.org/10.1007/978-1-0716-0585-1_24

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0584-4

  • Online ISBN: 978-1-0716-0585-1

  • eBook Packages: Springer Protocols

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