Abstract
Bioprinting cells with an electrically conductive bioink provides an opportunity to produce three-dimensional (3D) cell-laden constructs with the option of electrically stimulating cells in situ during and after tissue development. We and others have demonstrated the use of electrical stimulation (ES) to influence cell behavior and function for a more biomimetic approach to tissue engineering. Here, we detail a previously published method for 3D printing an electrically conductive bioink with human neural stem cells (hNSCs) that are subsequently differentiated. The differentiated tissue constructs comprise functional neurons and supporting neuroglia and are amenable to ES for the purposeful modulation of neural activity. Importantly, the method could be adapted to fabricate and stimulate neural and nonneural tissues from other cell types, with the potential to be applied for both research- and clinical-product development.
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References
Crook JM, Wallace G, Tomaskovic-Crook E (2015) The potential of induced pluripotent stem cells in models of neurological disorders: implications on future therapy. Expert Rev Neurother 15(3):295–304
Dean B (2009) Is schizophrenia the price of human central nervous system complexity? Aust N Z J Psychiatry 43:13–24
Gu Q, Tomaskovic-Crook E, Lozano R, Chen Y, Kapsa RM, Zhou Q et al (2016) Functional 3D neural mini-tissues from printed gel-based human neural stem cells. Adv Healthc Mater 5:1429–1438
Tomaskovic-Crook E, Zhang P, Ahtiainen A, Kaisvuo H, Lee CY, Beirne S et al (2019) Human neural tissues from neural stem cells using conductive biogel and printed polymer microelectrode arrays for 3D electrical stimulation. Adv Healthc Mater 8(15):1900425
Crook JM, Tomaskovic-Crook E (2017) Culturing and cryobanking human neural stem cells. In: Crook JM, Ludwig TE (eds) Stem cell banking: concepts and protocols, Methods in molecular biology, vol 1590. Human Press, New York
Acknowledgments
The authors wish to acknowledge funding from the Australian Research Council (ARC) Centre of Excellence Scheme (CE140100012).
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Tomaskovic-Crook, E., Crook, J.M. (2020). 3D Bioprinting Electrically Conductive Bioink with Human Neural Stem Cells for Human Neural Tissues. In: Crook, J.M. (eds) 3D Bioprinting. Methods in Molecular Biology, vol 2140. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0520-2_10
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DOI: https://doi.org/10.1007/978-1-0716-0520-2_10
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-0519-6
Online ISBN: 978-1-0716-0520-2
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