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Choice of Explant for Plant Genetic Transformation

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Biolistic DNA Delivery in Plants

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2124))

Abstract

Particle bombardment or biolistic transformation is an efficient, versatile method. This method does not need any vector for the gene transfer and is not dependent on the cell type, species, and genotype. The success of any transformation technique depends on the starting experimental materials or the explants. Here, we describe the factors that have influenced the choice of explants in biolistic transformation. Many general factors in the selection of explants in the development of transgenic plants are presented here. Therefore, this chapter provides extensive guidelines regarding the choice of explants for researchers working on various plant genetic transformation techniques.

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Acknowledgments

The authors gratefully acknowledge Department of Botany, DST PURSE PHASE II, UGC-CAS, The University of Burdwan for pursuing research activities for this work.

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Authors and Affiliations

  1. Department of Botany, UGC-Center of Advanced Study, The University of Burdwan, Golapbag, West Bengal, India

    Nibedita Chakraborty, Priyanka Chakraborty, Moutushi Sen & Rajib Bandopadhyay

  2. Department of Biotechnology, National Institute of Technology, Durgapur, West Bengal, India

    Nibedita Chakraborty & Moutushi Sen

  3. Department of Botany, Durgapur Government College, Durgapur, West Bengal, India

    Moutushi Sen

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  1. Nibedita Chakraborty
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  2. Priyanka Chakraborty
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  3. Moutushi Sen
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  4. Rajib Bandopadhyay
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Editors and Affiliations

  1. Department of Plant and Environmental Sciences, School of Health Research, Pee Dee Research and Education Center, Clemson University, Florence, SC, USA

    Sachin Rustgi

  2. Department of Genetics and Biochemistry, Clemson University, Clemson, SC, USA

    Hong Luo

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Chakraborty, N., Chakraborty, P., Sen, M., Bandopadhyay, R. (2020). Choice of Explant for Plant Genetic Transformation. In: Rustgi, S., Luo, H. (eds) Biolistic DNA Delivery in Plants. Methods in Molecular Biology, vol 2124. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0356-7_5

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  • DOI: https://doi.org/10.1007/978-1-0716-0356-7_5

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