Abstract
Microarray technique is used for the simultaneous detection of multiple pathogens infecting plants in a single reaction. This method uses virus-specific oligos immobilized on a membrane or glass slide as probe. The total RNA isolated from infected plant is converted into cDNA and amplified through PCR using pathogen-specific primers, labelled using suitable molecules for detection. The amplified and labelled products are then applied to the array and allowed for hybridization. After washing, array will be developed depending on the label used and result visualized using CCD with suitable software.
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Bhat, A.I., Rao, G.P. (2020). DNA Microarray for Detection of Plant Viruses. In: Characterization of Plant Viruses . Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0334-5_37
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DOI: https://doi.org/10.1007/978-1-0716-0334-5_37
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