Abstract
Monoclonal antibodies (MAb) are identical immunoglobulins targeted to a single epitope, generated from a single B-cell clone. These antibodies recognize and bind to a single epitope in an antigen. The steps involved in the generation of MAb include production of polyclonal antibodies against the antigen in a mouse. Instead of collecting antiserum (as in the case of polyclonal antibodies), in monoclonal antibody generation, the animal is sacrificed and cells that make antibodies (lymphocytes) are harvested. The lymphocytes are fused with myelomas, cancerous B cells, to create an immortalized hybrid that can undergo many passages in vitro. Hybridomas are then screened against the antigen to identify MAbs specific for the antigen. A protocol with different steps involved in the development of hybridoma and production of monoclonal antibody is discussed in this chapter.
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Bhat, A.I., Rao, G.P. (2020). Production of Monoclonal Antibody. In: Characterization of Plant Viruses . Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0334-5_29
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DOI: https://doi.org/10.1007/978-1-0716-0334-5_29
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