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Oriented Immobilization on Gold Nanoparticles of a Recombinant Therapeutic Zymogen

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Nanoparticles in Biology and Medicine

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2118))

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Abstract

Direct immobilization of functional proteins on gold nanoparticles (AuNPs) affects their structure and function. Changes may vary widely and range from strong inhibition to the enhancement of protein function. More often though the outcome of direct protein immobilization results in protein misfolding and the loss of protein activity. Additional complications arise when the protein being immobilized is a zymogen which requires and relies on additional protein–protein interactions to exert its function. Here we describe molecular design of a glutathione-S-transferase-Staphylokinase fusion protein (GST-SAK) and its conjugation to AuNPs. The multivalent AuNP-(GST-SAK)n complexes generated show plasminogen activation activity in vitro. The methods described are transferable and could be adapted for conjugation and functional analysis of other plasminogen activators, thrombolytic preparations or other functional enzymes.

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Author information

Authors and Affiliations

  1. Centre for Biomedical Sciences, School of Biological Sciences, Royal Holloway University of London, Egham, Surrey, UK

    Elina Dosadina, Celetia Agyeiwaa, William Ferreira & Simon Cutting

  2. Ashford and St. Peter’s Hospitals NHS Foundation Trust, Surrey, UK

    Abdullah Jibawi

  3. College of Science, School of Life Sciences, University of Lincoln, Brayford Pool, Lincoln, Lincolnshire, UK

    Enrico Ferrari

  4. Centre for Biomedical Sciences, Department of Biological Sciences, Royal Holloway University of London, Egham, Surrey, UK

    Mikhail Soloviev

Authors
  1. Elina Dosadina
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  2. Celetia Agyeiwaa
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  3. William Ferreira
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  4. Simon Cutting
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  5. Abdullah Jibawi
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  6. Enrico Ferrari
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  7. Mikhail Soloviev
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Corresponding author

Correspondence to Mikhail Soloviev .

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Editors and Affiliations

  1. College of Science, School of Life Sciences, University of Lincoln, Brayford Pool, Lincoln, Lincolnshire, UK

    Enrico Ferrari

  2. Centre for Biomedical Sciences, Department of Biological Sciences, Royal Holloway University of London, Egham, Surrey, UK

    Mikhail Soloviev

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Dosadina, E. et al. (2020). Oriented Immobilization on Gold Nanoparticles of a Recombinant Therapeutic Zymogen. In: Ferrari, E., Soloviev, M. (eds) Nanoparticles in Biology and Medicine. Methods in Molecular Biology, vol 2118. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0319-2_16

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  • DOI: https://doi.org/10.1007/978-1-0716-0319-2_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0318-5

  • Online ISBN: 978-1-0716-0319-2

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