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Methods for Screening Legume Crops for Abiotic Stress Tolerance through Physiological and Biochemical Approaches

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Legume Genomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2107))

Abstract

Legume crops are subjected to a wide range of abiotic stresses, which stimulate an array of physiological, biochemical, and molecular responses. However, different genotypes may exhibit significant variations between individual responses, which can determine their tolerance or susceptibility to these stresses. The present chapter suggests a broad range of assays that can help in understanding stress perception by plants at cellular and molecular levels. The genotypes may be sorted depending on their tolerance potential, by broadly analysing morphological, physiological, biochemical, and enzyme kinetics parameters. These assays are very beneficial in revealing the mechanism of stress perception and response in varied plant types, and have helped in discriminating contrasting genotypes. Here, we have described detailed protocols of assays which may be carried out to assess tolerance or susceptibility to abiotic stresses. The analysis, as a whole, can help researchers understand the effect of abiotic stresses on plant biochemical pathways, be it photosynthesis, redox homeostasis, metabolite perturbation, signaling, transcription, and translation. These protocols may be beneficial in identification of suitable donors for breeding programs, as well as for identifying promising candidate genes or pathways for developing stress tolerant legume crops through genetic engineering.

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Acknowledgments

AKS acknowledges funding support by Indian Council of Agricultural Research, New Delhi in the form of projects IXX12585 and IXX12644;Science and Engineering Research Board (SERB), Department of Science and Technology (DST), Government of India for the project under “Scheme for Young Scientists” (SB/YS/LS-255/2013). RS acknowledges DST-SERB for the National-Postdoctoral Fellowship (PDF/2016/000924), and DST, Govt. of India for funding under the Women Scientists Scheme-A (SR/WOS-A/LS-160/2018). MB acknowledges University Grant Commission (UGC), New Delhi for JRF.

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  1. ICAR-Indian Institute of Agricultural Biotechnology, Ranchi, India

    Ragini Sinha, Meenu Bala, Madan Kumar, Tilak Raj Sharma & Anil Kumar Singh

  2. Vinoba Bhave University, Hazaribagh, India

    Meenu Bala

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  1. Ragini Sinha
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  2. Meenu Bala
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  3. Madan Kumar
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  4. Tilak Raj Sharma
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  1. School of Computational and Integrative Sciences, Jawaharlal Nehru University, New Delhi, India

    Mukesh Jain

  2. Department of Life Sciences, School of Natural Sciences, Shiv Nadar University, Gautam Buddha Nagar, Uttar Pradesh, India

    Rohini Garg

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Sinha, R., Bala, M., Kumar, M., Sharma, T.R., Singh, A.K. (2020). Methods for Screening Legume Crops for Abiotic Stress Tolerance through Physiological and Biochemical Approaches. In: Jain, M., Garg, R. (eds) Legume Genomics. Methods in Molecular Biology, vol 2107. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0235-5_15

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  • DOI: https://doi.org/10.1007/978-1-0716-0235-5_15

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