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MAIT Cells pp 283–297Cite as

MAIT Cells in Type 1 Diabetes Mouse Models

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2098))

Abstract

MAIT cells are unconventional T cells expressing a semi-invariant αβ TCR, and they recognize bacterial metabolites via the highly conserved MR1 protein. MAIT cells interact with gut microbiota and literature reports alterations of gut homeostasis in type 1 diabetes (T1D), suggesting the involvement of MAIT cells in T1D. Since NOD mice is a well-established mouse model of T1D, MAIT cells were studied in these mice to evaluate their potential involvement in disease development. This chapter describes the material and methods required to characterize MAIT cells and to determine their function in T1D mouse models.

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Acknowledgments

We thank M. Salou for help in analysis of in vitro MAIT cell activation (5-OP-RU/fecal supernatant dilution); the mouse and Cybio facilities of the Cochin Institute. This work was supported by grants from INSERM, CNRS, Laboratoire d’Excellence consortium Inflamex ANR-11-IDEX-0005-02, and the Fondation pour la Recherche Médicale (FRM grant number DEQ20140329520, EQU201903007779 to A.L.), Fondation Francophone pour la Recherche sur le Diabète to A.L., EFSD/JDRF/Lilly to A.L., Aide aux Jeunes Diabétiques fellowship to I.N., Agence National de la Recherche (ANR-17-CE14-0002-01 Diab1MAIT to A.L.).

Author Contributions: I.N. and L.B. wrote the review and A.L. supervised the writing of the review.

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Authors and Affiliations

  1. INSERM U1016, Institut Cochin, Paris, France

    Isabelle Nel, Lucie Beaudoin & Agnès Lehuen

  2. CNRS, UMR8104, Paris, France

    Isabelle Nel, Lucie Beaudoin & Agnès Lehuen

  3. Université Paris Descartes, Laboratoire d’Excellence Inflamex, Sorbonne Paris Cité, Paris, France

    Isabelle Nel, Lucie Beaudoin & Agnès Lehuen

Authors
  1. Isabelle Nel
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  2. Lucie Beaudoin
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  3. Agnès Lehuen
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Corresponding author

Correspondence to Agnès Lehuen .

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Editors and Affiliations

  1. Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden, Clinical Immunology and Transfusion Medicine, Karolinska University Hospital, Stockholm, Sweden

    Helen Kaipe

  2. Department of Oncology-Pathology, Karolinska Institutet, Stockholm, Sweden

    Isabelle Magalhaes

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Nel, I., Beaudoin, L., Lehuen, A. (2020). MAIT Cells in Type 1 Diabetes Mouse Models. In: Kaipe, H., Magalhaes, I. (eds) MAIT Cells. Methods in Molecular Biology, vol 2098. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0207-2_18

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  • DOI: https://doi.org/10.1007/978-1-0716-0207-2_18

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0206-5

  • Online ISBN: 978-1-0716-0207-2

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