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Induction of Human Extraembryonic Mesoderm Cells from Naive Pluripotent Stem Cells

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Embryo Models In Vitro

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2767))

Abstract

The human extraembryonic mesoderm (EXM) is an important tissue in the postimplantation embryo which is specified before gastrulation in primates but not in rodents. EXM is mesenchymal and plays an important role in embryogenesis, including early erythropoiesis, and provides mechanical support to the developing embryo. Recently, it has been shown that self-renewing extraembryonic mesoderm cells (EXMCs) can be modeled in vitro by using human naive pluripotent stem cells. Here, we present a detailed step-by-step protocol to induce EXMCs from naive pluripotent stem cells in vitro.

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Acknowledgments

Research in the Pasque laboratory was supported by the Research Foundation-Fladers (FWO; FWO; Odysseus Return Grant G0F7716N to V.P.; FWO grants G0C9320N and G0B4420N to V.P.), the KU Leuven Research Fund (C1 GRANT C14/21/19 to V.P.), and FWO PhD fellowships to T.X.A.P. (11N3122N). We are also grateful to Susan Schlenner and the KU Leuven FACS core team for running the facility and especially Reena Chinaraj who helped us during flow cytometry experiments.

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Author notes

  1. Amitesh Panda and Thi Xuan Ai Pham contributed equally with all other contributors.

Authors and Affiliations

  1. Department of Development and Regeneration, Leuven Stem Cell Institute, Leuven Institute for Single-Cell Omics (LISCO), Leuven Cancer Institute, KU Leuven – University of Leuven, Leuven, Belgium

    Amitesh Panda, Thi Xuan Ai Pham, Sherif Khodeer & Vincent Pasque

Authors
  1. Amitesh Panda
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  2. Thi Xuan Ai Pham
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  3. Sherif Khodeer
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  4. Vincent Pasque
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Editor information

Editors and Affiliations

  1. Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA

    Magdalena Zernicka-Goetz

  2. Ottawa, ON, Canada

    Kursad Turksen

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Panda, A., Pham, T.X.A., Khodeer, S., Pasque, V. (2023). Induction of Human Extraembryonic Mesoderm Cells from Naive Pluripotent Stem Cells. In: Zernicka-Goetz, M., Turksen, K. (eds) Embryo Models In Vitro. Methods in Molecular Biology, vol 2767. Humana, New York, NY. https://doi.org/10.1007/7651_2023_483

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  • DOI: https://doi.org/10.1007/7651_2023_483

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3685-5

  • Online ISBN: 978-1-0716-3686-2

  • eBook Packages: Springer Protocols

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