Abstract
The characteristics of pluripotent cells have great potential for basic and clinical research and application. We describe the effect of normoxia or hypoxia regarding the proliferation and pluripotency of human iPS cells using colony number counting and real-time polymerase chain reaction (PCR). In addition, the function of hypoxia-inducible factors (HIFs) in human iPS cells under hypoxic conditions is evaluated in relation to the expression of pluripotency markers by siRNA and real-time PCR. Furthermore, we introduce the change of HIF-2α expression when signal transducer and activator of transcription 3 (STAT3) is suppressed by its inhibitor, Stattic or S31 201, using RT-PCR.
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Acknowledgments
This work was supported by Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science funded by the Ministry of Education, Science, Sports and Culture of Japan (grant number: 15K20407 to KS).
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Sugimoto, K., Hayashi, Y. (2020). The Characteristics of Human iPS Cells and siRNA Transfection Under Hypoxia. In: Nagy, A., Turksen, K. (eds) Induced Pluripotent Stem (iPS) Cells. Methods in Molecular Biology, vol 2454. Humana, New York, NY. https://doi.org/10.1007/7651_2020_299
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DOI: https://doi.org/10.1007/7651_2020_299
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Publisher Name: Humana, New York, NY
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