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Stem Cells and Aging pp 299–310Cite as

Histopathological and Behavioral Assessments of Aging Effects on Stem Cell Transplants in an Experimental Traumatic Brain Injury

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2045))

Abstract

Traumatic brain injury (TBI) displays cognitive and motor symptoms following the initial injury which can be exacerbated by secondary cell death. Aging contributes significantly to the morbidity of TBI, with higher rates of negative neurological and behaviors outcomes. In the recent study, young and aged animals were injected intravenously with human adipose-derived mesenchymal stem cells (hADSCs) (Tx), conditioned media (CM), or vehicle (unconditioned media) following TBI. The beneficial effects of hADSCs were analyzed using various molecular and behavioral techniques. More specially, DiR-labeled hADSCs were used to observe the biodistribution of the transplanted cells. In addition, a battery of behavior tests was conducted to evaluate the neuromotor function for each treatment group and various regions of the brain were analyzed utilizing Nissl, hematoxylin and eosin (H&E), and human nuclei (HuNu) staining. Finally, flow cytometry was also performed to determine the levels of various proteins in the spleen. Here, we discuss the protocols for characterizing the histopathological and behavioral effects of transplanted stem cells in an animal model of TBI, with an emphasis on the role of aging in the therapeutic outcomes.

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Authors and Affiliations

  1. Department of Neurosurgery and Brain Repair, Center of Excellence for Aging and Brain Repair, University of South Florida Morsani College of Medicine, Tampa, FL, USA

    Jea-Young Lee, Roger Lin, Hung Nguyen, M. Grant Liska, Trenton Lippert & Yuji Kaneko

  2. Center of Excellence for Aging & Brain Repair, Morsani College of Medicine, University of South Florida, 12901 Bruce B. Downs Blvd., MDC 78, Tampa, FL, 33612, USA

    Cesar V. Borlongan

  3. Department of Neurosurgery and Brain Repair, Morsani College of Medicine, University of South Florida, Tampa, FL, 33612, USA

    Cesar V. Borlongan

Authors
  1. Jea-Young Lee
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  2. Roger Lin
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  3. Hung Nguyen
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  4. M. Grant Liska
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  5. Trenton Lippert
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  6. Yuji Kaneko
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  7. Cesar V. Borlongan
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Corresponding author

Correspondence to Cesar V. Borlongan .

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Editors and Affiliations

  1. Ottawa, ON, Canada

    Kursad Turksen

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Lee, JY. et al. (2018). Histopathological and Behavioral Assessments of Aging Effects on Stem Cell Transplants in an Experimental Traumatic Brain Injury. In: Turksen, K. (eds) Stem Cells and Aging . Methods in Molecular Biology, vol 2045. Humana, New York, NY. https://doi.org/10.1007/7651_2018_121

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  • DOI: https://doi.org/10.1007/7651_2018_121

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9712-1

  • Online ISBN: 978-1-4939-9713-8

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