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Targeted Proteomics Driven Verification of Biomarker Candidates Associated with Breast Cancer Aggressiveness

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Tissue Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1788))

Abstract

Breast cancer is the most common and molecularly well-characterized malignant cancer in women; however, its progression to metastatic cancer remains lethal for 78% of patients within 5 years of diagnosis. Identifying novel markers in high risk patients using quantitative methods is essential to overcome genetic, inter-tumor, and intra-tumor variability, and to translate novel findings into cancer diagnosis and treatment. Using untargeted proteomics, we recently identified 13 proteins associated with some key factors of breast cancer aggressiveness: estrogen receptors, tumor grade, and lymph node status. Here we verified these findings in a set of 96 tumors using targeted proteomics based on selected reaction monitoring with mTRAQ labeling (mTRAQ-SRM). This study highlights a panel of gene products that could contribute to breast cancer aggressiveness and metastasis, and can help develop more precise breast cancer treatments.

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Abbreviations

DDA:

Data-dependent acquisition

ER:

Estrogen receptor

G1:

Tumor grade 1

G3:

Tumor grade 3

iTRAQ:

Isobaric tags for relative and absolute quantitation

MIDAS™:

MRM initiated detection and sequencing

mTRAQ:

Mass differential tags for relative and absolute quantification

mTRAQ-SRM:

Selected reaction monitoring with mTRAQ labeling

PR:

Progesterone receptor

SRM:

Selected reaction monitoring

TEAB:

Triethylammonium bicarbonate

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Acknowledgments

We would like to thank Rudolf Nenutil for his pathological guidance. We also thank Parhom Towfighi (UCSF Medical Centre) for his work on editing for the book. This work was supported by Czech Science Foundation (Project No. 17-05957S).

No conflict of interests.

Author information

Authors and Affiliations

  1. Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic

    Iva Procházková & Pavel Bouchal

  2. Masaryk Memorial Cancer Institute, Regional Centre for Applied Molecular Oncology, Brno, Czech Republic

    Iva Procházková

  3. Department of Molecular Pathology and Biology, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic

    Juraj Lenčo

Authors
  1. Iva Procházková
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  2. Juraj Lenčo
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  3. Pavel Bouchal
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Corresponding author

Correspondence to Pavel Bouchal .

Editor information

Editors and Affiliations

  1. Professor, Department of Surgery Medicine and Pediatrics Director, Kidney-Pancreas Transplant Program, University of California San Francisco, San Francisco, California, USA

    Minnie M. Sarwal

  2. Asst. Professor, Department of Surgery, University of California San Francisco, San Francisco, California, USA

    Tara K. Sigdel

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Procházková, I., Lenčo, J., Bouchal, P. (2017). Targeted Proteomics Driven Verification of Biomarker Candidates Associated with Breast Cancer Aggressiveness. In: Sarwal, M., Sigdel, T. (eds) Tissue Proteomics. Methods in Molecular Biology, vol 1788. Humana Press, New York, NY. https://doi.org/10.1007/7651_2017_111

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  • DOI: https://doi.org/10.1007/7651_2017_111

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7852-6

  • Online ISBN: 978-1-4939-7854-0

  • eBook Packages: Springer Protocols

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