Abstract
In this paper, an analytical method for the analysis of molecular lipids in algae samples is reported. The sample preparation is based on a modified Folch extraction, and the analysis is carried out with ultrahigh performance liquid chromatography combined with mass spectrometry (UPLC-MS). For further characterization of lipids, MS/MS analyses are carried out utilizing either a separate instrument (e.g., LTQ-Orbitrap mass spectrometer) or simultaneous fragmentation with the same instrument. Throughput of the method is over 100 samples/d. The repeatability is good, and the relative standard deviation of spiked samples is <15%.
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Acknowledgments
The authors thank Ulla Lahtinen and Airi Hyrkäs for excellent technical assistance in preparing extracts of algal samples.
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Nygren, H., Seppänen-Laakso, T., Rischer, H. (2017). Liquid Chromatography-Mass Spectrometry (LC-MS)-Based Analysis of Molecular Lipids in Algae Samples. In: Spilling, K. (eds) Biofuels from Algae. Methods in Molecular Biology, vol 1980. Humana, New York, NY. https://doi.org/10.1007/7651_2017_108
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DOI: https://doi.org/10.1007/7651_2017_108
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