Abstract
The culture of organoids has represented a significant advancement in the gastrointestinal research field. Previous research studies have described the oncogenic transformation of human intestinal and mouse gastric organoids. Here we detail the protocol for the oncogenic transformation and orthotopic transplantation of human-derived gastric organoids.
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References
McCracken KW et al (2014) Modelling human development and disease in pluripotent stem-cell-derived gastric organoids. Nature 516:400–404
Sato T et al (2009) Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche. Nature 459:262–265
Schumacher MA et al (2015) The use of murine-derived fundic organoids in studies of gastric physiology. J Physiol (Lond) 593:1809–1827
Bertaux-Skeirik N et al (2015) CD44 plays a functional role in Helicobacter pylori-induced epithelial cell proliferation. PLoS Pathog 11, e1004663
Huch M et al (2013) In vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration. Nature 494:247–250
Huch M et al (2015) Long-term culture of genome-stable bipotent stem cells from adult human liver. Cell 160:299–312
Dedhia PH (2016) Organoid models of human gastrointestinal development and disease. Gastroenterology. doi:https://doi.org/10.1053/j.gastro.2015.12.042
Sato T et al (2011) Long-term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett’s epithelium. Gastroenterology 141:1762–1772
Boj SF et al (2015) Organoid models of human and mouse ductal pancreatic cancer. Cell 160:324–328
Neal JT, Kuo CJ (2016) Organoids models for neoplastic transformation. Annu Rev Pathol 11:199–220
Matano M et al (2015) Modeling colorectal cancer using CRISPR-Cas9-mediated engineering of human intestinal organoids. Nat Med 21:256–262
Nadauld LD et al (2014) Metastatic tumor evolution and organoid modeling implicate TGFBR2 as a cancer driver in diffuse gastric cancer. Genome Biol 15:428
Li X et al (2014) Oncogenic transformation of diverse gastrointestinal tissues in primary organoid culture. Nat Med 20:769–777
Acknowledgements
This work was supported by NIH 1R01DK083402 grant and College of Medicine Bridge Funding Program (Zavros), NIH 5T32GM105526, Albert J. Ryan Fellowship, and Dean’s Fellowship (Bertaux-Skeirik).
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Bertaux-Skeirik, N., Centeno, J., Gao, J., Gabre, J., Zavros, Y. (2016). Oncogenic Transformation of Human-Derived Gastric Organoids. In: Turksen, K. (eds) Organoids. Methods in Molecular Biology, vol 1576. Humana, New York, NY. https://doi.org/10.1007/7651_2016_4
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DOI: https://doi.org/10.1007/7651_2016_4
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-7616-4
Online ISBN: 978-1-4939-7617-1
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