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Dopaminergic Differentiation of Human Embryonic Stem Cells on PA6-Derived Adipocytes

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Embryonic Stem Cell Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1341))

Abstract

Human embryonic stem cells (hESCs) are a promising source for cell replacement therapies. Parkinson’s disease is one of the candidate diseases for the cell replacement therapy since the motor manifestations of the disease are associated with the loss of dopaminergic neurons in the substantia nigra pars compacta. Stromal cell-derived inducing activity (SDIA) is the most commonly used method for the dopaminergic differentiation of hESCs. This chapter describes a simple, reliable, and scalable dopaminergic induction method of hESCs using PA6-derived adipocytes. Coculturing hESCs with PA6-derived adipocytes markedly reduces the variable outcomes among experiments. Moreover, the colony differentiation step of this method can also be used for the dopaminergic induction of mouse embryonic stem cells and NTERA2 cells as well.

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Acknowledgements

This study was supported by AXREGEN (EU Framework 7 project, 214003), the Linnaeus grant Bagadilico (Swedish Research Council, No. 349-2007-8626), FNRS-Sinergia (Swiss National Foundation, No. 125408), and Strategic Research Area MultiPark (Multidisciplinary Research in Parkinson’s Disease) at Lund University.

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Authors and Affiliations

  1. Department of Experimental Medical Science, Faculty of Medicine, Lund University, 22184, Lund, Sweden

    M. Oktar Guloglu & Anna Larsen

Authors
  1. M. Oktar Guloglu
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  2. Anna Larsen
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Corresponding author

Correspondence to M. Oktar Guloglu .

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Editors and Affiliations

  1. Ottawa Hospital Research Institute, Ottawa, Ontario, Canada

    Kursad Turksen

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© 2015 Springer Science+Business Media New York

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Guloglu, M.O., Larsen, A. (2015). Dopaminergic Differentiation of Human Embryonic Stem Cells on PA6-Derived Adipocytes. In: Turksen, K. (eds) Embryonic Stem Cell Protocols. Methods in Molecular Biology, vol 1341. Humana Press, New York, NY. https://doi.org/10.1007/7651_2015_235

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  • DOI: https://doi.org/10.1007/7651_2015_235

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2953-5

  • Online ISBN: 978-1-4939-2954-2

  • eBook Packages: Springer Protocols

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